摘要
建立了水产品中3种喹诺酮类（恩诺沙星、环丙沙星、氧氟沙星）兽药多残留的新型Qu ECh ERS前处理技术结合酶联免疫快速检测的分析方法。基本流程为样品经90%乙腈（1%乙酸）均质提取离心后,上清液依次用C18净化、静置沉淀蛋白后用于测定,该前处理方法灵敏、快速、经济、实用性强。测试曲线线性范围为0.107~177.896ng/m L,检出限为0.0087μg/kg,IC50值为4.34ng/m L。样品批内和批间平均回收率分别为94.10%、93.70%,批内变异系数为4.09%~8.41%,批间变异系数2.78%~7.99%。检测结果与HPLC的相关系数R2=0.9897,表明酶联免疫检测方法及样品前处理方法适合喹诺酮类药物多残留检测。
A method was developed for the simultaneous determination of 3 Fluoroquinolones（FQs） （ enrofloxacin, ciprofioxacin, ofloxacin）residues including enrofloxacin, ciprofloxacin, ofloxacin in seafood samples by new QuEChERS and Enzyme-linked Immunosorbent Assay（ ELISA）.The approach involved the extraction of sample with acetonitrile containing 1% （v/v）acetic acid, and the supernatant liquid was directly purified with C18, meanwhile,was used for the precipitation of protein. The method was proved to be sensitive, rapid, precise, economical and applied.The calibration curve should be virtually linear in the range of 0.107- 177.896ng/mL, the limit of detection（LOD） was 0.0087μg/kg, and IC50 value was 4.34ng/mhAverage recovery rate was 94.10% and 93.70% separately in same batch and different batch,coefficient of variation was 4.09%-8.41% and 2.？8%-7.99% separately in same batch and different batch.The results showed good coefficient with HPLC（ R2 = 0.9897）, which proved to be suitable for the screening of seafood samples for the presence of FQs.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第1期292-298,共7页
Science and Technology of Food Industry
基金
广州市珠江科技新星专项(2013J2200080)
国家星火计划(2012GA780001
2013GA780035)
NSFC-广东联合基金(U1301214)
广东省自然科学基金(S2013030013338)
教育部博士点基金(20114404130002)
广东省科技计划项目(重点专项)(2012A020100002
2010A032000001-4)
广州市科技企业孵化器发展专项(2012Ss-P204)
