摘要
为了解天山马鹿南山种群的遗传多样性,选用5对微卫星标记引物,利用PCR扩增,用非变性聚丙稀酰胺凝胶电泳、银染法显色,对天山马鹿南山山区冬季采集的255份马鹿粪便进行了检测,统计了该种群的基因频率(P)、有效等位基因数(Ne)、群体杂合度(He)、多态信息含量(PIC)。结果表明:255份粪便DNA分属111个体;种群平均等位基因4.40±0.90;平均有效等位基因数3.7391±0.1998;平均多态信息含量0.6825±0.0083;平均期望杂合度0.7257±0.0001;平均观察杂合度0.894±0.0214。整个种群没显著偏离Hardy-Weinberg平衡,种群固定系数Fis为-0.232。从此可见,天山马鹿南山种群具有比较丰富的遗传多样性。
To reveal the genetic diversity of the Tianshan Red Deer in Nanshan population,the genetic polymorphisms of 5 microsatellites in 2 Nanshan groups were studied by means of PCR polyacrylamide gel electrophoresis and silver staining. Number of alleles,average effective number of alleles（ E） and average rates of homozygote were counted. According to allele frequencies of 5 microsatellites,polymorphism information content（ PIC）,mean heterozygosity（ h） were calculated for each population. The result showed that these markers were highly polymorphic loci with rich genetic diversity in the Nanshan populations. The total of 255 fecal DNA of the samples in 2 groups was assigned to 111 individuals. The mean number of allele and effective allele（ Ne） were 4. 40± 0. 90 and 3. 7391 ± 0. 1998,respectively. The average polymorphic information content（ PIC） were 0. 6825 ±0. 0083,and the mean observed heterozygosity（ Ho） and expected heterozygosity（ He） were 0. 7257 ± 0. 0001 and 0. 894 ± 0. 0214,respectively. The population was not significantly deviated from Hardy- Weinberg Equilibrium（ HWE） and the inbreeding coefficient was- 0. 232. It was suggested that the genetic diversity of the Tianshan Red Deer in Nanshan population was still higher.
出处
《干旱区资源与环境》
CSSCI
CSCD
北大核心
2014年第12期132-137,共6页
Journal of Arid Land Resources and Environment
基金
国家自然科学基金项目(课题号:30660025
31060152)
新疆维吾尔自治区自然科学基金项目(课题号:2010211A02)
新疆大学自治区重点学科-动物学科资助
关键词
遗传多样性
天山马鹿
微卫星
新疆南山山区
genetic diversity
Tianshan Red Deer
microsatellite
Nanshan mountain in Xinjiang
