摘要
土壤中解磷微生物能够增加磷酸钙的溶解性,从而提高植物对磷的利用效率。采集草坪根际盐碱化土壤,采用涂布平板法进行菌种初筛,并对菌株进行生物学特性研究。结果表明,所得解磷菌株是一株解磷效果较好的真菌(记作F1305),其发酵液中的有效磷质量浓度为280.35mg/L;解磷圈直径与菌落直径之比(D/d)达到1.78;最适碳源为麦芽糖,乳糖培养基上生长最差;对于所供4种氮源,菌株F1305在酵母浸膏上过于疯长,在亚硝酸钠上几乎不生长,最适氮源为酵母浸膏,硝酸钠次之;4种温度及pH条件下,菌株F1305均可生长,最适温度为35℃,最适pH为7.0。发酵条件的优化组合试验得到菌株F1305的最适解磷条件,即温度为35℃,pH为6.0,碳氮比为20∶1(质量比),转速设置为100r/min运行12h、然后130r/min运行24h、最后100r/min运行12h。
Phosphate-solubilizing microorganisms in soil could increase the solubility of calcium phosphate,so as to improve the utilization of phosphorus in plants.The lawn rhizosphere saline alkali soil was collected to screen bacterial strain by using spread plate method,and the biological characteristics of screened strains were studied.The results showed that：the screened fungi （record as F1305） had perfect phosphorolysis characteristics,the phosphorus concentration in fermentation broth of F1305 was 280.35 mg/L.Diameter ratio of phosphate dissolving circle and colony circle （D/d） could reach 1.78.The optimum carbon source for F1305 was maltose,and the worst was lactose cultivation base.F1305 grew too fast in the yeast extract and hardly to grow in sodium ntrite.Of the four nitrogen sources,yeast extract was the optimum nitrogen source and the following one was nitrate sodium.The most appropriate temperature and pH for F1305 growth was 35 ℃ and 7.0 respectively.The optimal phosphate solubilisation conditions were 35 ℃,pH=6.0,C/N=20： 1,running at 100 r/min of rotation speed for the first 12 h,then 130 r/min for the next 24 h and finally 100 r/min for the last 12 h.
出处
《环境污染与防治》
CAS
CSCD
北大核心
2014年第12期50-53,58,共5页
Environmental Pollution & Control
基金
山东省自然科学基金资助项目(No.ZR2013EEL001)
山东省自然科学基金联合专项(No.ZR2012CL05)
国家级大学生创新训练计划项目(No.201310449124)
滨州市环境工程技术重点实验室开放基金资助项目(No.bzhks201401)
关键词
解磷真菌
生物学特性
解磷特性
phosphate-solubilizing fungi
biological property
phosphate-solubilizing characteristics
