摘要
为定位水稻发芽期和芽期耐冷性的加性QTL和上位性QTL,本试验以粳稻品种空育131和东农422构建的F7代重组自交系（RIL）190个家系为作图群体,利用104个SSR标记构建遗传连锁图谱,利用完备区间作图法分别对低温发芽力和芽期耐冷性进行QTL定位并分析其加性效应和上位性效应。结果检测到控制芽期耐冷性的1个加性QTL位于4号染色体上,贡献率为16.84%;17个控制低温发芽力的加性QTL分别位于第1、2、3、6、7、9、12染色体上,贡献率为5.64%~35.67%;控制芽期耐冷性的上位性QTL2对,累积贡献率19.3%;控制发芽期耐冷性的上位性QTL 33对,各性状累计贡献率介于18.35%~91.08%,分别控制第7、10、11、15天的发芽率和平均发芽天数的表达,累积贡献率分别为87.88%,87.38%,91.08%,78.68%和18.35%。上位性在水稻发芽期和芽期耐冷性遗传中作用重大,因此,在分子标记育种中加性QTL和上位性QTL是很重要的。
The objective of this study was to investigate the additive effects and the epistatic effects of QTLs for the cold tolerance of germination period and the bud bursting period. In this study, F7 population including 190 indivi- duals derived from a cross between Dongnong 422 and Kongyu 131 were used for constructing genetic linkage map, which was constructed based on 104 SSR molecular markers. We located quantitative trait loci for cold tolerance to low temperature germinability （LTG） and cold tolerance at bud-growth stage （CTP） by using inclusive composite interval mapping method. The results as follows：One QTL with additive effects for CTP was located on chromosome 4, which explained 16.84% of the phenotypic variance. Seventeen QTL with additive effects on chromosome 1,2,3, 6,7,9 and 12 which were associated with LTG were detected which explained the range of the observed phenotypic variance from 5.64% to 35.67%. Two pairs of CTP QTLs with epistatic effects were found in the RIL, accounting for 19.3% of the general phenotypic variation, and thirty-three pairs of LTG QTLs with epistatie effects were detec- ted,accounting for 18.35% ,87.88% ,87.38% ,91.08% and 78.68% ,respectively of the general phenotypic vari- ation. These QTLs represented the mean length of incubation time and the germination rate at about 7d, 10d, l ld and 15d, respectively. The results indicated that the additive effects and the epistatic effects factor played an impor- tant role in the cold tolerance of germination period and the bud bursting period in rice. Therefore, QTLs with addi- tive effects and epistatic effects should be considered in the improvement of rice breeding.
出处
《作物杂志》
CAS
CSCD
北大核心
2014年第6期44-51,共8页
Crops
基金
国家科技支撑计划(2011BAD35B02-01
2013BAD20B04-1S
2011BAD16B11)
关键词
水稻
重组自交系
芽期
发芽期
冷胁迫
QTL分析
Rice
RIL population
Germination period
Bud bursting period
Cold stress
QTL analysis
