摘要
目的:检测男性免疫性不育患者精浆白介素6(IL-6)和可溶性细胞粘附分子1(s ICAM-1)水平,探讨炎症细胞因子与男性免疫性不育的关系。方法:按精子膜表面抗体免疫珠试验结果将临床疑为不育症患者分为免疫性不育组(Ig A或Ig G抗体黏附的精子数≥50%)41例、其他原因不育A组(10%≤Ig A或Ig G抗体黏附的精子数<50%)37例,其他原因不育B组(Ig A或Ig G抗体黏附的精子数<10%)45例。按白细胞过氧化物酶染色结果将免疫性不育患者分为免疫性白细胞阳性组和免疫性白细胞阴性组;选择同期因女方因素不孕前来检查的生育力评估正常的健康男性31例作为对照组。统计分析各组炎症因子精浆表达水平及精浆主要参数的差异。精液液化时间通过肉眼观察和显微镜进行识别判读,精子浓度及活力用计算机辅助精子分析系统,精子存活率采用低渗膨胀实验(HOS),抗精子抗体采用免疫珠试验(IBT);白细胞过氧化物酶染色采用正甲苯胺法,IL-6和s ICAM-1的含量测定采用酶联免疫吸附(ELISA)法。结果:各不育组与对照组比较,精子存活率和前向运动精子百分率有统计学差异(P<0.05或P<0.01)。免疫性不育组、其他原因不育A组、其他原因不育B组和对照组精浆IL-6(ng/L)、s ICAM-1(ng/ml)水平分别为37.92±17.01、89.15±41.82,22.23±13.77、67.81±33.24,18.75±14.32、53.25±27.09,9.47±5.76、19.46±9.77。各不育组与对照组比较,IL-6、s ICAM-1均有非常显著性差异(P<0.01),免疫性不育组与其他原因不育两组分别比较差异均有统计学意义(P<0.05或P<0.01)。免疫性白细胞阳性组和免疫性白细胞阴性组精浆IL-6(ng/L)、s ICAM-1(ng/ml)的水平分别为49.25±21.46、104.36±46.41和31.38±15.54、80.38±35.52,两者比较均有统计学差异(P<0.05)。结论:男性精浆中IL-6和s ICAM-1升高可能与免疫性不育有关。
Objective： To detect the expressions of interleukin-6 （IL-6） and soluble intercellular adhesion molecule-1 （sICAM-1 ） in the seminal plasma of infertile men and explore the role of inflammatory cytokines in male immune infertility. Methods ： Based on the results of the sperm-bound antibody immunobead test, 123 males with clinically suspected infertility were divided into an immune infertility group （ n = 41 ）, other infertility group A （ n = 37 ）, and other infertility group B （ n = 45 ）. The immune infertility patients were further subdivided into a leukocyte-positive and a leukocyte-negative group according to the results of leukocyte peroxidase staining. The control group included 31 males confirmed to be fertile in the clinic. Statistical analyses were conducted on the differences in inflammatory cytokines expressions and main parameters in the seminal plasma among different groups. The seminal liquefaction time was measured by visual and microscopic observation, sperm concentration and motility detected using the computer-assisted sperm analysis system, sperm viability determined by hypotonic swelling assay, and the expression levels of IL-6 and sICAM-1 measured by ELISA. Results： The infertility groups showed significantly lowers penn viability （P 〈 0.05） and progressive motility （P 〈 0.01 ） than the fertile control, but no remarkable differences from the latter in sperm concentration （P 〉 0.05 ） and semen liquefaction time （P 〉 0.05 ）. No statistically significant differences were observed in seminal parameters between the immune infertility group and other infertility groups （P 〉 0.05）. The IL-6 and sICAM-1 levels in the seminal plasma were extremely significantly higher in the im- mune infertility group （ [ 37.92± 17.01 ] ng/L and [ 89.15 ± 41.82 ] ng/ml）, other infertility group A （ [ 22.23 ± 13.77 ] ng/L and [67.81 ± 33.24] ng/ml）, and other infertility group B （ [ 18.75 ±14.32] ng/L and [53.25 ± 27.09] ng/ml） than in the normal control group （ [9.47 ± 5.76] ng/L and [ 19.46 ＋ 9.77] ng/ml） （P 〈0.01 ）, with remarkable differences between the immune infertility group and the other two infertility groups （ P 〈 0.05 ）. The leukocyte-positive patients showed significantly increased levels of IL-6 （ [49.25 ±21.46] ng/L） and sICAM-1 （ [ 104.36 ±46.41 ] ng/ml） as compared with the leukocyte-negative ones （[31.38 ± 15.54] ng/Land [80.38 ± 35.52] ng/ml） （bothP〈0.05）. Conclusion： IL-6 and sICAM-1 in the seminal plasma are involved in male immune infertility.
出处
《中华男科学杂志》
CAS
CSCD
2014年第12期1098-1102,共5页
National Journal of Andrology
关键词
免疫性不育
白介素6
可溶性细胞黏附分子1
male immune infertility
interleukin-6 （IL-6）
soluble intercellular adhesion molecule-1 （sICAM-1）
