摘要
在液态发酵条件下,采用单因素实验确定了Aspergillus niger PZ331产异淀粉酶的最适碳源和氮源,分别为蔗糖和硝酸铵。在上述基础上利用Plackett-Burman设计对影响产异淀粉酶的因素进行评价,并筛选出硝酸铵、接种量、培养温度3个主要因素;继而利用响应面设计优化了最佳硝酸铵浓度、接种量和培养温度。最终确定了最优培养条件为:蔗糖10 g/L,硝酸铵10 g/L,磷酸氢二钾3 g/L,硫酸亚铁0.01 g/L,硫酸镁1 g/L,起始p H值4.2;接种量2%(孢子浓度为107cfu/m L),30℃培养72 h,酶活达137.3μ/m L;比基础培养基的提高了1.71倍左右。
Based on liquid fermentation,the optimum carbon and nitrogen of isoamylase for Aspergillus niger PZ331 were obtained through the single factor experiment,which were sucrose and NH4NO3,respectively. Some process factors of isoamylase production were evaluated by Plackett-Burrman design,and three significant factors（ NH4NO3,inoculation amount and temperature） were obtained. Response surface methodology was further used to optimize the three critical internal factors. The results showed that the optimum culture medium（ g / L） of isoamylase was composed of 10 NH4NO3,10 sucrose,3 K2HPO4,0. 01 Fe SO4,1 Mg SO4,initial p H 4. 2;Under the above conditions,Aspergillus niger PZ331 was cultured under 30℃ for 72 h with inoculation amount of 2%（ spore concentrations of 107 cfu / m L）,the maximum yield of isoamylase was 137. 3 μ / m L,which increased by 1. 71- fold.
出处
《生物学杂志》
CAS
CSCD
2014年第6期60-63,78,共5页
Journal of Biology
基金
安徽省教育厅重点项目(KJ2012A246)
合肥学院生物工程院级重点学科
合肥学院发酵工艺重点实验室资助
