摘要
目的：建立芪黄通秘软胶囊中黄芪甲苷含量的测定方法。方法：采用高效液相色谱-蒸发光散射检测器法（HPLC-ELSD）,色谱柱为C18柱（250mm×4.6mm,5μm）,流动相为甲醇-水（75∶25）,流速为1.0mL·min-1,柱温为35℃,漂移管温度为105℃,载气流速为2.0L·min-1。结果：该方法专属性良好,黄芪甲苷线性范围为1.832~5.496μg（r=0.999）,加样回收率为99.70%,RSD为1.12%,耐用性良好。结论：该方法重现性好、灵敏、准确,可作为芪黄通秘软胶囊质量的控制方法。
Objective： To establish an method for determing astragaloside Ⅳ in Qihuangtongmi soft capsules. Methods： The H PLC-ELSD method was conducted on a C18 column （250mm ×4.6mm, 5mm） with methanol：water （75： 25） as mobile phase. The flow rate of mobile phase was 1.0 mL/min. The column temperature was at 35℃. The temperature of drift tube for measurement was 105℃ and the flow rate of air was 2.0 L · min-1. Results：The method is highly specific, the linear ranges of astragaloside IV was 1. 832～ 5. 496μg（ r = 0. 999, n： 5）. The average recoveries of astragaloside Ⅳ were 99.70% with corresponding RSD of 1.12%. Conclusion：The method is sensitive, accurate with a good reproducibility and is reliable for quality control of Qihuangtongmi soft capsules.
出处
《亚太传统医药》
2015年第1期23-25,共3页
Asia-Pacific Traditional Medicine
