期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MiR-29c模拟物对骨肉瘤细胞侵袭和迁移能力的影响

The effect of miR- 29c mimics on invasion and migration of osteosarcoma cell lines
下载PDF
导出
摘要 目的:观察miR-29c在具有不同转移能力的F4和F5M2细胞系中的表达差异;研究miR-29c模拟物对骨肉瘤细胞侵袭和迁移能力的影响。方法:利用qRT-PCR技术检测F4与F5M2间miR-29c的表达差异;将骨肉瘤细胞F5M2分为3组,运用细胞转染技术分别对两组转染miR-29c模拟物、模拟物阴性对照,第3组不作处理,随后用qRT-PCR技术检测3组间miR-29c的表达差异。Transwell法检测3组不同处理的F5M2细胞的侵袭和迁移能力是否存在差异。结果:MiR-29c在F4和F5M2细胞系中表达差异显著,miR-29c在高转移性的F5M2细胞中表达水平显著下降。与阴性对照组和空白组相比,转染miR-29c模拟物的F5M2细胞侵袭和迁移能力均明显减弱,且差异具有统计学意义(P<0.05)。结论:MiR-29c在高转移性骨肉瘤细胞中低表达,且miR-29c模拟物具有抑制骨肉瘤细胞侵袭和转移的作用。MiR-29c有可能作为骨肉瘤转移基因治疗的一个新靶点。 Objective：To observe the expression of miR-29c in osteosarcoma cell lines with high-metastatic po-tential F5M2 and low-metastatic potential F4,and to investigate the effect of miR-29c mimics on invasion and mi-gration abilities of OS cell lines. Methods：qRT-PCR was performed to detect the expressions of miR-29c in F4 and F5M2. Then F5M2 cells were divided into three groups,which were respectively transfected with miR-29c mim-ics（experiment group）,mimics NC（control group）,and nothing（blank group）. The expression of miR-29c in three groups were detected by using qRT-PCR. Transwell analysis was preformed to demonstrate the invasion and migra-tion abilities of OS cell lines. Results：The miR-29c expression level in F5M2 cells was lower comparing with that in F4 cells. The invasion and migration abilities of OS cells in experiment group comparing with control group and blank group which were confirmed by one-way ANOVA analysis. Conclusion：MiR-29c was down-regulated in OS cell lines with high-metastatic potential. MiR-29c mimics could inhibit the invasion and migration of OS cells.
作者 董川 闫康 张开亮 韩康 路遥 王猛 李楠 马保安
机构地区 第四军医大学唐都医院骨科全军骨科中心暨骨肿瘤研究所
出处 《现代肿瘤医学》 CAS 2015年第1期8-11,共4页 Journal of Modern Oncology
基金 国家自然科学基金面上项目(编号:81072194)
关键词 骨肉瘤 侵袭 迁移 Transwell实验 转移相关miRNA miR-29c osteosarcoma invasion migration Transwell test metastasis associated miRNA miR - 29c
分类号 R730 [医药卫生—肿瘤] R738.1 [医药卫生—肿瘤]
  • 相关文献

参考文献28

  • 1Bielack S, Cattle D, Casali PG. Osteosarcoma:ESMO clinical rec- ommendations for diagnosis, treatment and follow - up [ J ]. Ann Onco1,2009 ,20 ( suppl 4 ) : 137 - 139.
  • 2Geller DS, Gorlick R. Osteosarcoma : a review of diagnosis, manage- ment, and treatment strategies[ J]. C lin Adv Hematol 0ncol,2010, 8(10) :705 -718.
  • 3Carrie D, Bielaek S. Osteosareoma lung metastases detection and principles of muhimodal therapy [ J ]. Cancer Treat Res, 2009, 152:165 - 184.
  • 4Chou J, Shahi P, Werb Z. microRNA - mediated regulation of the tumor microenvironment [ J ]. Cell Cycle, 2013,12 ( 20 ) : 3262 - 3271.
  • 5Sehmitt M J, Margue C, Behrmann I, et al. MiRNA - 29 : a microR- NA family with tumor - suppressing and immune - modulating properties[ J]. Curr Mol Med,2013,13 (4) :572 - 585.
  • 6Chen X, Yang TT, Wang W, et al. Establishment and characteriza- tion of human osteosareomu cell lines with different pulmonary me- tastatic potentials[J]. Cytotechnology ,2009,61 ( 1 - 2 ) :37 - 44.
  • 7Bartel DP. MicroRNAs:target recognition and regulatory functions [ J]. Cell ,2009,136(2) :215 - 233.
  • 8Zhao H, Guo M,Zhao G, et al. miR -183 inhibits the metastasis of osteosarcoma via downregulation of the expression of Ezrin in FSM2 cells[J]. Int J Mol Med,2012,30(5) :1013 - 1020.
  • 9Zhao G, Cai C, Yang T, et al. MicroRNA - 221 induces cell survival and cisplatin resistance through PI3K/Akt pathway in human osteo- sarcoma[ J]. PLoS One,2013,8( 1 ) : e53906.
  • 10Zhao H, Ma B, Wang Y, et al. miR - 34a inhibits the metastasis of osteosarcoma cells by repressing the expression of CD44 [ J ]. On- col Rep ,2013,29 (3) : 1027 - 1036.

二级参考文献31

  • 1孟颖,汤华,强冉,刘民,李欣.miRNA对肿瘤细胞活性影响的探讨[J].临床和实验医学杂志,2006,5(2):137-138. 被引量：6
  • 2Bartel DP.MicroRNAs:genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
  • 3Esquela-Kerscher A,Slack FJ.Oncomirs-microRNAs with a role in cancer[J].Nat Rev Cancer,2006,6(4):259-269.
  • 4Lu J,Getz G,Miska EA,et al.MicroRNA expression profiles classi-fy human cancers[J].Nature,2005,435(7043):834-838.
  • 5Calin GA,Liu CG,Sevignani G,et al.MicroRNA profiling reveals distinct signatures in B cell chronic lymphocytic leukemias[J].Proc Natl Acad Sci U S A 2004,101(32):11755-11760.
  • 6Tsuchiya H,Tomita K,Mori Y,et al.Marginal excision for osteo-sarcoma with caffeine assisted chemotherapy[J].Clin Orthop Relat Res,1999,(358):27-35.
  • 7Lee RC.Feinbaum RL.Ambros V.The C.elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J].Cell,1993,75(5):843-854.
  • 8Calin GA,Sevignani C,Dumitru CD,et al.Human microRNA genes are frequently located at fragile sites and genomic regions in-volved in cancers[J].Proc Natl Acad Sci U S A,2004,101(9):2999-3004.
  • 9Calin GA,Dumitru GD,Shimizu M,et al.Frequent deletions and down-regulation of micro-RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia[J].Proc Natl Acad Sci U S A,2002,99(24):15524-15529.
  • 10Johnson SM,Grosshans H,Shingara J,et al.RAS is regulated by the let-7 microRNA family[J].Cell.2005,120(5):635-647.

共引文献17

现代肿瘤医学
2015年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部