摘要
目的:荧光定量聚合酶链反应(FQ-PCR)技术检测乳腺癌易感基因1(breast cancer susceptibility gene 1,BRCA1)、乳腺癌易感基因2的表达及其与临床病理特征之间的关系,探讨其在散在乳腺癌诊断中的应用。方法:采用FQ-PCR法,并以甘油醛-3-磷酸脱氢酶(GAPDH)为内对照测定30名健康女性体检者、55例良性乳腺疾病患者、96例乳腺癌患者外周血中BRCA1和BRCA2的表达量。结果:乳腺癌患者BRCA1和BRCA2的mRNA显著低于健康女性体检者和良性乳腺疾病患者。健康女性体检者和良性乳腺疾病患者BRCA1和BRCA2的mRNA无显著性差异。GAPDH和BRCA1/BRCA2比值在三组间无显著性差异。乳腺癌BRCA2 mRNA显著高于BRCA1。BRCA1和BRCA2 mRNA之间有微弱的相关性(r=0.378,P<0.01)。BRCA2 mRNA和BRCA1/BRCA2比值与临床病理(ER和PR、组织学分级)存在相关性。BRCA1基因表达与临床病理不存在相关性。结论:采用FQ-PCR检测BRCA1和BRCA2基因表达水平,可有效监测散在乳腺癌的诊断、疗效和转移。
Objective：To evaluate the clinical application of fluorescent quantification polymerase chain reaction （ FQ-PCR）to detect BRCA1 and BRCA2 in the diagnosis of sporadic breast cancer and the relationship of their ex-pression with clinicopathological factors. Methods：BRCA1 and BRCA2 mRNA expression in the serum of 96 patients with sporadic invasive ductal carcinoma were quantified by FQ-PCR. GAPDH was used as internal control. Results：BRCA1 and BRCA2 mRNA was significantly lower than those in health women and patients with benign breast dis-ease. There was no significant difference in BRCA1 and BRCA2 mRNA between health women and patients with be-nign breast disease. There was no significant difference in BRCA1/BRCA2 and GAPDH among three groups. BRCA2 mRNA was significantly higher than BRCA1. There was a weak but significant correlation between BRCA1 and BRCA2 mRNA（r=0. 378,p〈0. 01）. There was significant association between BRCA2 mRNA expression and clin-icopathological factors（ ER and PR,histological grade）. There was no correlation between BRCA1 mRNA expression and clinicopathological factors such as menstrual status,tumor size,lymph node status,estrogen and progesterone re-ceptor status,and histological grade. There was difference between BRCA1/2 and clinicopathological factors（ ER and PR,histological grade）. Conclusion：FQ-PCR is a rapid method for quantitating BRCA1 and BRCA2. It also gives objective evidence to the diagnosis,therapy,metastasis of breast cancer.
出处
《现代肿瘤医学》
CAS
2015年第1期59-61,共3页
Journal of Modern Oncology
基金
四川省杰出青年基金资助项目(编号:2007-5-345)
