摘要
目的：建立乳制品中的VC液相色谱分析方法.方法：样品经偏磷酸沉淀蛋白后,滤纸过滤,以0.25％偏磷酸溶液为流动相;经C18反相色谱柱分离,用紫外检测器检测,外标法定量.结果：该方法操作简便、快速,具有良好的准确度和精密度,回收率93.6％～103.9％、相对标准偏差2.56％～4.05％、定量检出限1 mg/kg.
A method for determining vitamin C （VC） in dairy products by high performance liquid chromatography （HPLC） is described. Samples were added with metaphosphoric acid to precipitate proteins, filtered through filter paper, separated on a C18 chromatographic column with 0.25% metaphosphoric acid solution as mobile phase, detected with a UV detector, and quantified by an external standard method. The method proved simple and rapid with good accuracy and precision. The recovery of VC ranged between 93.6 % and 103.9% with relative standard deviation of 2.56% to 4.05%. The detection limit was 1 mg/kg.
出处
《乳业科学与技术》
2014年第5期14-16,共3页
JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY
基金
"十二五"国家科技支撑计划项目(2012BAK17B16)
关键词
液相色谱法
乳制品
VC
检测
high performance liquid chromatography （HPLC）
dairy products
vitamin C （VC）
determination
