摘要
目的探讨白虎加人参汤对2型糖尿病大鼠胰岛素抵抗模型的影响及其作用。方法通过尾静脉注射四氧嘧啶联合高脂饲料喂养制备2型糖尿病大鼠胰岛素抵抗模型,观察白虎加人参汤对大鼠空腹血糖(FPG)、空腹胰岛素(FINS)、胰岛素敏感指数(ISI)、血清总胆固醇(TC)和甘油三酯(TG)水平及葡萄糖转运蛋白4(GLUT4)、胰岛素受体(Ins R)基因表达的影响。结果与模型组比较,白虎加人参汤低、高剂量均可显著降低糖尿病大鼠FPG、FINS、TC和TG含量,显著升高ISI(P<0.05,P<0.01),明显增加骨骼肌GLUT4、肝细胞膜Ins R蛋白表达,显著上调骨骼肌GLUT4 m RNA、肝脏Ins R m RNA表达(P<0.01)。结论白虎加人参汤可降低糖尿病大鼠FPG、FINS、TC和TG含量,显著升高ISI,对2型糖尿病胰岛素抵抗模型大鼠胰岛功能有明显保护作用,其机制可能与调控骨骼肌GLUT4、肝细胞膜Ins R m RNA和蛋白表达水平,维持胰岛细胞的正常结构和功能密切相关。
Objective To investigate the the effect of White Tiger Decoction with Ginseng on the insulin resistance of type 2 diabetes rats, and to explore its protective mechanism. Methods Tail vein injection of alloxan combined with high fat diet feeding was given to the rats to induce a pathological model of insulin resistance of type 2 diabetes. The influence of White Tiger Decoction with Ginseng on the fasting plasma glucose（FPG）,fasting insulin（FINS）,insulin sensitivity index（ISI）, serum total cholesterol（TC） and triglyceride（TG） levels as well as the gene expression of glucose transporter 4（GLUT4）and insulin receptor（Ins R）were evaluated. Results Compared with the model group,the low-dose and high-dose of White Tiger Decoction with Ginseng could significantly reduce the the content of FPG,FINS, TC and TG of the diabetic rats, increase the ISI（P〈0.05 or P〈0.01）, and up-regulate the protein and m RNA expression levels of skeletal muscle GLUT4 and liver cell membrane Ins R（P〈0.01）. Conclusion White Tiger Decoction with Ginseng exerts markedly protective effect on the pancreatic islet function of type 2 diabetes rats through reducing the contents of FPG, FINS, TC and TG and increasing the ISI. And its mechanism may be related to regulating the m RNA and protein expressions of skeletal muscle GLUT4 and liver cell membrane Ins R, and to recovering the normal structure and functions of pancreatic islets.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2014年第6期683-687,共5页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省高等学校高层次人才项目(A1-AFD004132A13)
