摘要
该研究以探索油菜秸秆厌氧消化产甲烷潜力为目的,利用一组高效纤维素分解产甲烷菌群在CSTR厌氧反应器内分解定量油菜秸秆41 d,通过监测厌氧发酵过程中的甲烷生产效率,以及纤维素酶活性等指标,评价油菜秸秆厌氧发酵产甲烷的能力。结果表明:油菜秸秆在发酵菌群的作用下能够大量产生沼气,50 g干秸秆厌氧发酵后总产气量为13 200 m L,产气效率达到264 m L/g。在发酵过程中,秸秆纤维素被有效分解,纤维素酶活性和半纤维素酶活性分别达0.63、0.81 U/m L。纤维素酶活性与沼气产量具有良好的相关性,相关性系数达到0.95,表明秸秆纤维素类厌氧发酵产沼气体系内,具有良好纤维素酶活性的菌群对甲烷生产具有重要的意义。
The article aims at exploring potential methane production of rape straw by anaerobic digestion. It uses a set of efficient cellulolytic bacterium consortium which continuously decompose rape straw in anaerobic CSTR for 41 days. The activity of methane production from rape straw by anaerobic digestion was evaluated by monitoring methane production efficiency,cellulase activity and other indicators during anaerobic fermentation process. Results showed that rape straw can produce large amount of biogas by fermentation bacterium consortium,and 50 g dry straw can produce 13 200 m L biogas during fermentation process,biogas production can reach 264 m L/g. The cellulose of straw is effectively decomposed during the fermentation process,with cellulase activity and hemicellulase activity up to 0.63 U/m L and 0.81 U/m L respectively.Cellulase activity has a good correlation with biogas production,indicating that cellulose bacterium consortium has an important effect on methane production in biogas fermentation system of straw cellulose.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2014年第12期193-198,共6页
Environmental Science & Technology
基金
国家自然基金面上项目(31370506)
车用生物燃料技术国家重点实验室开放基金资助项目(2013022)
关键词
油菜秸秆
厌氧发酵
沼气
酶活
rape stalk
anaerobic fermentation
biogas
enzyme activity
