摘要
目的：探讨细胞周期与细胞凋亡相关蛋白p53、bcl－2、Ki－67和细胞凋亡在角化棘皮瘤（Keratoacanthoma，KA）和高分化鳞状细胞癌（Well－differentiated squamous cell carcinoma ，WDSCC）中的表达及其意义。方法应用免疫组化技术和末端特异性DNA标记技术检测44例角化棘皮瘤、20例高分化鳞状细胞癌标本中p53、bcl－2、Ki－67和细胞凋亡的表达情况。结果 KA增生期、成熟期和消退期p53阳性表达率分别为：22．23％、26．18％和6．52％，低于WDSCC 41．82％。 KA各期分别与WDSCC阳性率比较，差异有统计学意义；Ki－67的表达强度和模式同p53相似，p53表达率与Ki－67表达率呈正相关（ r＝0．986，P＜0．001）；KA中仅1例基底层见bcl－2弱阳性表达，WDSCC中2例表达呈弱阳性；KA的平均凋亡率（21．72％）明显高于WDSCC（9．93％），两者比较差异有统计学意义，细胞凋亡率与增殖率（Ki－67）的表达呈负相关（r＝－0．824，P＜0．001）。结论增生和凋亡同时存在于KA中，早期增生占优势，而消退期凋亡占优势，最终导致KA的自然消退。 p53、Ki－67以及细胞凋亡的表达对临床鉴别KA与WDSCC有一定参考价值。
Objective To explore the expressions and significances of p 53、bcl-2、Ki-67 and apopto-sis in keratoacanthoma（KA）and well differentiated squamous cell carcinoma （WDSCC）.Methods The expres-sions of p53、bcl-2、Ki-67 were detected by immunohistochemical technique in 44 cases of KA and 20 cases of WDSCC.Apoptosis was identified by using terminal deoxynucleotidyl transfers （ TdT） -mediated dUTP -biotin nick and labelling（TUNEL）method.Results The positive expressive rates of p53 in proliferative、mature and re-gressive of KA were 22.23%、26.18% and 6.52%, which was lower than the positive rate of WDSCC （41.82%）.Significant differences were found between every period of KA with WDSCC in p 53 expression;The intensity of expression and pattern were similar in KA of Ki -67 and p53.There were a positive correlation be-tween p53 and Ki-67 expression rate（r=0.986,P〈0.001）.The bcl-2 staining showed weak expression in 1 case in WDSCC and 2 cases in KA ,but only a few positive tumor cells was limited to basal cell layer in KA .The average apoptosis rate in KA was 21.72%,which was apparently higher than in WDSCC （9.925%）.There were a negative correlation between the apoptosis rate and proliferation rate of Ki -67（r=-0.824,P〈0.001）.Con-clusion The proliferation and apoptosis in KA can coexist .However ,the apoptosis will occupy a dominance posi-tion in regressive phase ,which results in the regression of tumor .The expressions of p53、Ki-67 and apoptosis would be a certain significance in differentiated KA and WDSCC .
出处
《实用肿瘤学杂志》
CAS
2014年第6期485-491,共7页
Practical Oncology Journal
基金
黑龙江省自然科学基金项目（D201278）
哈尔滨市科技创新人才（2012RFXYS047）
