摘要
为了检测烯脂酰辅酶A水合酶短链1(ECHS1)的甲基化状态,以同源比对克隆的方法获得荷斯坦奶牛ECHS1部分片段的序列,首先预测甲基化数目,然后采用重亚硫酸盐转化测序(BS-Seq)的方法检测了所获得片段的甲基化状态。结果表明,该试验成功获得了ECHS1 459 bp的序列,其C+G含量为53.88%,含有28个Cp G岛,其中1号、2号、9号、11号、13号、14号、17号、22号、24号、27号位点所有个体都发生了甲基化。
In order to detect the methylation status of enoyl-CoA hydratase short chain 1 （ECHS1）, the sequence fragments of ECHS1 was obtained for Holstein cows by homologous cloning. The number of methylation was firstly predicted, and then methylation status of the obtained sequence fragments was detected by method of sequencing of bisulfite conversion. The results showed that fragments of ECHS1 with the size of 459 bp were obtained. C＋G content was 53.88%. The fragments contained 28 CpGs islands and all individuals at sites 1, 2, 9, 11, 13, 14, 17, 22, 24 and 27 had been methylated.
出处
《畜牧与饲料科学》
2014年第12期7-9,12,共4页
Animal Husbandry and Feed Science
基金
内蒙古农牧业科学院创新基金项目(2011CXJJM05-5)
