摘要
以患石榴枯萎病植株的5份根际土壤为试材,采用梯度稀释涂布法分离到放线菌,继而利用对峙培养法筛选石榴枯萎病、芋头黑斑病病原甘薯长喙壳拮抗菌并测试其抗菌谱,以期为利用对环境友好的生防放线菌有效防治蒙自甘薯长喙壳病害奠定基础。结果表明:19株石榴根际土壤放线菌中的9株拮抗石榴枯萎病、芋头黑腐病病原甘薯长喙壳及小桐子果腐病Bgf-1,其中菌株DCAA-12具有高拮抗活性且生长速率较高,具备蒙自石榴、芋头甘薯长喙壳病害防治及其病壤修复的应用前景。
Taking 5 samples of pomegranate rhizosphere soil as materials, and had separated isolate actinomycetes by gradient dilution coating method and screen antagonistic strains on the pathogen of Pomegranate Wilt, Taro Black Rot together with testing their angonistic spectrum by confrontation culture method,in order to lay foundation for effectively control the plant disease resulted by C. fimbriata in Mengzi county exploring eco-friendly soil actinomycetes. The results showed that 9 strains of 19 strains actinomyeetes from pomegranate rhizosphere soil could inhibit the pathogens of Pomegranate Wilt;Taro Black Spot and Jatropha fruit rot, additional DCAA-12 with higher antagonistic activity and growth rate, which could be used to control the diseases of pomegranate and taro caused by C. fimbriata and reverse diseased soil.
出处
《北方园艺》
CAS
北大核心
2015年第1期117-119,共3页
Northern Horticulture
基金
云南省应用基础研究资助项目(2013FZ125)
云南省教育厅科研基金资助项目(2012Y453)
红河学院硕士点植物保护一级学科建设资助项目
红河学院2011年大学生科技创新基金资助项目(SZ1112)
关键词
石榴枯萎病
芋头黑腐病
甘薯长喙壳
拮抗放线菌
Pomegranate Wilt
Taro Black Spot
Ceratocystis fimbriata
antagonistic actinomycetes
