摘要
目的：建立天仙子药材中东莨菪碱和莨菪碱的高效液相测定方法。方法采用 Symmetrix ODS-H色谱柱，以乙腈-0．1％三氟乙酸水（14∶86）为流动相，检测波长210 nm，流速1．0 mL／min，测定天仙子药材中东莨菪碱和莨菪碱的含量。结果氢溴酸东莨菪碱和硫酸阿托品的线性范围分别为4．02～513．64μg／mL （r ＝0．9999）和4．00～512．00μg／mL （r＝0．9998），平均回收率分别为98．41％和98．79％。结论 HPLC法测定准确、可控，能快速、简便地控制天仙子药材的质量。
Objective To establish quality control methods for determining active ingredients of the total al-kaloids from Hyoscyami semen .Methods The determination of scopolamine and hyoscyamine in H . semen was performed on Symmetrix ODS-H column and mobile phase consisted of acetonitrile-0.1% tri-fluoroacetic acid （14∶86）.The flow rate was 1.0 mL/min and the UV detective wavelength was set at 210 nm. Results The linear ranges of scopolamine and hyoscyamine were 4.02-513.64μg/mL （r=0.999 9）and 4.00-512.00μg/mL （r=0.999 8）,and the average recoveries were 98.41% and 98.79% respectively.Conclusion The method could be easily used to evaluate the quality of H .semen ,quick,accurate and controllable.
出处
《新疆医科大学学报》
CAS
2015年第1期64-66,共3页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区科技支撑项目(201433104)
