摘要
目的：比较避光与见光提取的枳术丸挥发油中主要成分含量的差异性.方法：采用水蒸气蒸馏法提取挥发油,挥发油提取器分别采用不包裹（见光）或者用黑布包裹（避光）提取相同时间.采用HPLC比较2种挥发油中苍术酮和白术内酯Ⅰ,Ⅱ,Ⅲ的含量差异,流动相水（A）-乙腈（B）梯度洗脱（0～22 min,55％～64％ B;22 ～28 min,64％ ～ 64％ B;28 ～48 min,64％ ～ 100％ B）,检测波长220,275 nm.结果：见光与避光提取的挥发油得率分别为1.03％和1.05％.见光提取的挥发油与避光提取的挥发油相比,苍术酮含量显著降低,分别为73.01,100.3 mg·g-1;白术内酯Ⅰ显著升高,分别为0.757 2,0.354 1mg·g-1;白术内酯Ⅱ差异不明显,分别为2.026,2.158 mg·g-1;白术内酯Ⅲ显著升高,分别为0.412 4,0.321 9 mg·g-1.结论：见光与避光提取的枳术丸挥发油总量无明显差异,但主要成分含量存在明显差异.提示在研究复方物质基础时,应注意实验过程中可能引起的内在成分变化.
Objective： To compare content differences of main components in volatile oil from Zhizhu pills extracted in light and dark conditions. Method： Volatile oil in Zhizhu pills was obtained by steam distillation, one extracting volatile oil device was not wrapped to get oil exposed to the sun light oil （light condition oil） and the another device was wrapped with black cloth to get oil keeping out of the sun light （dark condition oil） , these two devices were used to extract volatile oil for the same time. Comparison of contents of atractylone, atractylenolide Ⅰ , Ⅱ ,Ⅲ in these two kinds of volatile oil from Zhizhu pills were determined by HPLC, mobile phase consisted of water （A） -acetonitrile （B） for gradient elution of （0-22 min, 55%-64% B; 22-28min, 64% B; 2848 min, 64%-100% B） , detection wavelength were 220 nm and 275 nm. Result： Yields of volatile oils extracted in dark and light conditions were 1.05% and 1.03% , respectively. The content of atractylone in light condition oil was significantly lower than that in dark condition oil, which were 73.01 mg ·g-1 and 100.3 mg·g-1,respectively. The content of atractylenolide I in light condition oil was much higher than that in dark condition oil, which were 0. 757 2 mg·g-1 and 0. 354 1 mg·g-1, respectively. There was no significant difference in the content of atractylenolide Ⅱ between these two kinds of oils, which were 2. 026 mg·g-1 and 2. 158 mg·g-1, respectively. The content of atractylenolide Ⅲ in light condition oil was obviously higher than that in dark condition oil, which were 0.412 4 mg·g-1 and 0. 321 9 mg·g-1, respectively. Conclusion： No significant differences of yields of volatile oil are existed in these two extracted methods, but main ingredients aremarkedly different. It is suggested that inherent compounds change caused by experimental process should be avoided in constituent studies of Chinese prescription.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第1期49-52,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央级公益性科研院所基本科研业务费专项(YZ-1317)
关键词
枳术丸
挥发油
光照
白术
枳实
苍术酮
白术内酯类
Zhizhu pills
volatile oil
illumination
Atractylodis Macrocephalae Rhizoma
Aurantii Fructus Immaturus
atractylone
atractylenolides
