摘要
蚕丝织物的水解老化过程会伴随着丝蛋白中氨基酸的氧化和流失,分别以浓盐酸和Na OH固体配制4种p H值的酸、碱溶液对蚕丝织物进行水解老化处理,以强酸(p H 2)、强碱(p H 12)溶液水解处理蚕丝织物的老化程度较严重。采用柱前衍生-反相高效液相色谱技术测定不同p H值溶液水解老化蚕丝织物丝蛋白的氨基酸含量,分析酪氨酸和天门冬氨酸含量与蚕丝织物水解老化程度的关系:强酸、强碱溶液处理10 d,天门冬氨酸含量均明显下降,并与老化处理时间呈现较好的线性关系,且4种p H值溶液处理蚕丝织物丝蛋白中的天门冬氨酸含量与断裂强力保留率之间可形成一定的函数对应关系;酪氨酸含量增加,与处理时间之间无明显规律。因此认为,天门冬氨酸含量比酪氨酸含量更适合作为蚕丝织物水解老化程度的评估指标。此外,丝蛋白中的谷氨酸与天门冬氨酸类似且同属于酸性氨基酸,而测试结果中的天门冬氨酸含量与谷氨酸含量呈正相关关系,也为利用天门冬氨酸含量表征蚕丝织物水解老化程度的良好效果提供了佐证。
Aging process of silk fabrics under hydrolytic condition is accompanied by oxidation and loss of amino acids.In this study,concentrated hydrochloric acid and solid Na OH were used to prepare acidic and alkaline solutions with 4p H values which were further used to hydrolyze silk fabrics. The results indicated that the aging degree of silk samples treated with strong acid（ p H 2） or strong base（ p H 12） was higher than that of other treatments. The amino acid contents of hydrolyzed silk samples were also measured by reversed phase high performance liquid chromatography with pre-column derivatization（ RP-HPLC） for analyzing the relationship of tyrosine and aspartic acid contents with aging degree of silk samples. When the treatment time by strong acid or strong base was prolonged to 10 days,the percentage of aspartic acid in the treated silk samples decreased obviously. There was a good linear relationship between aspartic acid content and the treatment time. Moreover,among treatments by acidic or alkaline solution with all 4 p H values,aspartic acid content in proteins of the treated silk fabrics displayed a functional corresponding relationship with retention rate of breaking strength,while no regularity was found between tryosine content and the treatment time. Therefore,aspartic acid content was a more suitable indicator of silk aging degree under hydrolysis than tyrosine content. In addition,in silk protein,glutamic acid is similar with aspartic acid,both of which are of acidic amino acids. In our measurement result,aspartic acid content was positively correlated with glutamic acid,which further verified the good effect of using aspartic acid content to charac-terize the aging degree of silk fabrics.
出处
《蚕业科学》
CAS
CSCD
北大核心
2014年第6期1084-1091,共8页
ACTA SERICOLOGICA SINICA
基金
浙江省文物局文物保护科技项目(No.2012005)
关键词
蚕丝织品
老化程度
酸碱水解
酪氨酸
天门冬氨酸
Silk fabric
Aging degree
Acid or alkaline hydrolysis
Tyrosine
Aspartic acid
