摘要
对1 432株海洋和极地来源的细菌进行抗南方根结线虫的离体筛选,并对其中活性较好的一株菌进行菌种鉴定并通过盆栽实验验证其在实际生防中的效果.设计诱导系统抗性实验,测定植物防御酶活的变化,初步研究细菌的抗虫机制.结果显示,从1 432株细菌离体筛选得到了9株具有抗南方根结线虫的细菌,其中南极土壤来源的菌株1A00316在离体和活体中均表现出较好的抗性,其生防效果达到71.67%,经鉴定该菌株为恶臭假单胞菌(Pseudomonas putida).诱导番茄系统抗性实验结果显示,经菌株1A00316发酵液处理后的番茄3种防御酶苯丙氨酸解氨酶(PAL)、多酚氧化酶(PPO)以及过氧化物酶(POD)的酶活均显著提高,分析数据认为该菌株的菌体可诱导番茄系统抗性.本研究表明恶臭假单胞菌1A00316抗南方根结线虫效果较好,可作为微生物制剂应用于实际生防中;该菌不仅有直接抗虫作用,还存在间接抗虫机制,其多重抗虫机制也具有进一步的研究价值.
Meloidogyne incongnita causes serious damage to crops. Nowadays, attention has been paid to the side effects of chemical insecticides control. It is important to isolate the antagonistic microbe against root knot nemtaode from microbial resources. In this study 1 432 marine and polar isolates were screened against nematode to identify 9 strains with high nematocidal activity to study its biocontrol effect on nematode by pot experiments. Among them the strain 1A00316 isolated from the South Pole showed good inhibition on M. incongnita in vitro and in pot experiment, with biocontrol efficiency on nematodes as 71.67%. The experiment of induced systemic resistance （ISR） was conducted to study the possible mechanisms of 1A00316 against M. incongnita by determining the enzymes. Strain identification showed the strain 1A00316 belonged to Pseudomonas putida. The ISR mediated by strain 1A00316 in tomato clearly showed an improvement of activity of three defense enzymes： phenylalnineammonialyase （PAL）, polyphenol oxidase （PPO） and peroxidase （POD） in tomato plants. The result indicated strain 1A00316 itself could induce systematic resistance to tomato and enhance their resistance against nematode. This research showed that P. putida strain 1A00316 has good resistance to M. incongnita, of both direct and induced systemic resistance, therefore can be used as microbial agents.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2014年第6期1046-1051,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家重点基础研究发展计划项目(2013CB127504)资助~~
