摘要
目的：建立测定吊筋药中栀子苷含量的高效液相色谱方法。方法采用 Phenomenex LUNA-C18柱（4.6 mm×250 mm，5μm），以乙腈∶水（12∶88）为流动相，流速1.0 ml/min，柱温30℃，检测波长238 nm。结果栀子苷在0.049～0.980 mg/ml浓度范围内有良好线性关系（r＝0.9999），平均加样回收率为99.1%，RSD 为1.66%。结论本方法简便，快速，准确，重复性好，可用于吊筋药中栀子苷的含量测定。
Objective To establish a high performance liquid chromatography （HPLC） method for the determination of geniposide in Diaojinyao powder. Methods The HPLC system consisted of the Fortis-C18 （4.6 mm × 250 mm, 5μm） column, the mobile phase consisted of HCN∶H2O （12∶88） , the flow rate was 1.0 ml/min, the column temperature was 30℃, The UV detector was set at 238 nm. Results The linear response range was 0.049～0.980mg/ml （ r=0.999 9）. The average recovery of geniposide was 99.1%, and RSD was 1.66%. Conclusion The method is simple, rapid, accurate and repeatable. It can be applied in determination of Geniposide in Diaojinyao Powder.
出处
《国际中医中药杂志》
2015年第1期70-72,共3页
International Journal of Traditional Chinese Medicine
基金
湖北省“2011计划”资助项日（2011）
关键词
栀子苷
吊筋药
高效液相色谱法
Geniposide
Diaojinyao powder
High performance liquid chromatography
