摘要
目的:取跟腱病大鼠健侧相关穴位给予电针处理,探讨缪刺法对跟腱病组织修复的影响,为缪刺治疗跟腱病提供理论依据。方法:从36只2月龄雄性大鼠中选出6只,设为空白对照组(K组,n=6),对其余大鼠行跟腱病造模后随机分为阳性对照组(Y组,n=18)、缪刺组(M组,n=12)。Y组随机分为Y0组、Y1组和Y2组,各6只,M组随机分为M1组和M2组,各6只。造模3周后,M组取健侧阳陵泉、足三里、承山以及太溪,给予电针治疗,每日1次,每次15分钟,10天为1疗程,共2个疗程,2疗程间隔2天;K组和Y组大鼠不予任何干预,均自由饮食。造模3周后分别处死K组和Y0组大鼠,第1疗程和第2疗程后分别处死Y1、M1组和Y2、M2组大鼠,标本用HE染色观察组织学结构改变,作跟腱内成纤维细胞计数后用Microsoft Excel统计软件分析。用免疫组织化学染色法检测TGF-β1表达情况,并与组织学观察及统计结果作比较,以评价跟腱病组织学恢复情况。结果:1第1疗程后M1组成纤维细胞数量显著高于Y1组,差异具显著;第2疗程后M2组成纤维细胞数量显著低于Y2组,差异具显著。2TGF-β1:K组未见TGF-β1表达,各期阳性对照组有弱阳性表达,而M组有TGF-β1阳性表达。结论:缪刺能有效地促进跟腱病的修复,其机制可能是刺激TGF-β1的合成,提高TGF-β1的活性,进而促进了成纤维细胞增殖及细胞外基质合成与胶原纤维的重塑。
Objective： To observe the effect of electro- acupuncture on Achilles tendinopathy by stimulating some related points of rat＇s healthy lower limb and to provide information for healthy side acupuncture treatment of achilles tendinopathy. Methods： Six male SD（ 2months old） rats were at random drown out from thirty-six rats as blank contrast group（ K group）. The models of rat＇s achilles tendinopathy were set up by 1% collagenase injection into the left achilles tendons of the other thirty rats.The model rats were divided into controlled group（ Y group）,healthy side acupuncture group（ M group）. Group Y was divided into three subgroups（ Y0,Y1,Y2group） and group M were respectively divided into two groups（ M1,M2group）. After three weeks of setting up the model,M1 and M2group were treated respectively by electro-acupuncturing the healthy limbs points Yanglingquan,Zusanli,Chengshan,Taixi,once a day. Each time lasted 15 minutes. A treating process was 10 days. After resting two days,the second treating process started. Group K and Y had no treatment. Group K and group Y0 were executed after three weeks of setting up the model. Groups Y1,M1 and Y2,M2 were executed at the end of the first and second treating process. For all achilles tendons specimen,histological and immunohistochemical observation were obtained. The number of the fibroblasts was counted and statistically analysis by Microsoft excel. The effect of transforming growth factor betar1 was obtained and compared with the histological observation to study the recovery of achilles tendinopathy. Results： 1After the fist treating process,the fibroblast counts of the group M1 were significantly higher than the group Y1 and showed significantly difference between the group M1 and Y1. After the second treating process,the fibroblast counts of the group M2 were significantly lower than the group Y2 and showed significantly difference between the groups M2 and Y2. 2TGF-β1was not detected in the group K,TGF-β1expressed in the group Y and expressed strongly in the group M. Conclusions： Healthy side acupuncture treatment can improve effectively the recovery of achilles tendinopathy. It can enhance fibroblast proliferation and promote collagen synthesis by stimulating the synthesis of TGF-β1and activating TGF-β1.
出处
《四川中医》
2014年第12期52-55,共4页
Journal of Sichuan of Traditional Chinese Medicine
关键词
跟腱病
电针
缪刺
组织学观察
化生长因子β1
Achilles tendinopathy
Electro - acupuncture
Healthy side acupuncture treatment
Histology observation
Transforming growth factor betal
