摘要
目的建立超高效液相色谱（UPLC）同时测定热炎宁颗粒中大黄素、咖啡酸、绿原酸、野黄芩苷、木犀草素、虎杖苷6种成分的方法。方法采用UPLC法,Acquity UPLC HSS C18色谱柱（100 mm×2.1 mm,1.7μm）;流动相为[甲醇–乙腈（35∶65）]–0.1%磷酸水溶液为流动相,梯度洗脱,体积流量0.5 m L/min,分段变波长测定,柱温35℃;进样量1.0μL。结果 6种成分在选定的范围内线性关系良好（r≥0.999 3）;平均加样回收率在99.08%102.31%,RSD值均小于2.53%（n=6）。结论采用UPLC法对热炎宁颗粒中大黄素、咖啡酸、绿原酸、野黄芩苷、木犀草素、虎杖苷6种成分进行测定,方法操作快速,高效,准确,可用于热炎宁颗粒的质量控制。
Objective To develop a UPLC method for simultaneous determination of emodin, caffeic acid, chlorogenic acid, scutellarin, luteolin, and polydatin in Reyanning Granules. Methods UPLC chromatography was used. The chromatographic separation was carried out on an Acquity UPLC HSS C18 column（100 mm × 2.1 mm, 1.7 μm） with [methanol- acetonitrile（21∶79）]- 0.1% phosphoric acid as mobile phases at the flow rate of 0.5 m L/min for gradient elution. Detection with variable wavelength was used, and the column temperature was 35 ℃ with injection volume of 1.0 μL. Results There were good separations for six components in the specific ranges（r≥0.999 3）. The average recovery was in the range of 99.08% — 102.31% with RSD value less than 2.53%（n = 6）. Conclusion Simultaneous determination method of emodin, caffeic acid, chlorogenic acid, scutellarin, luteolin, and polydatin in Reyanning Granules by UPLC is established, and is accurate, sensitive, credible, and repeatable which can be applied to the quality control for Reyanning Granules.
出处
《现代药物与临床》
CAS
2014年第12期1365-1368,共4页
Drugs & Clinic
基金
天津市中医药管理局中医中西医结合科研项目(13140)
