摘要
要建立了反向微乳毛细管电泳（MEEKC）在线富集技术灵敏检测多环芳烃的方法。采用大体积进样-pH动态连接-扫集微乳毛细管电泳法（LVSS-DypH-MEEKC）对于常规条件下很难分离的6种强亲脂性的多环芳烃中性分子进行富集分离。结果表明,在反相电压下,当微乳液的组成为：2.4%（w/w）SDS、0.6%（w/w）正辛烷、6.6%（w/w）正丁醇、20 mmol/L Na H2PO4缓冲液（pH 2.2）;进HCB时间为20 s（16 kPa）,进样时间为80 s（16 kPa）时,富集效果良好,富集倍数在25～80倍之间,在27 min内实现了对多环芳烃化合物的灵敏检测。将本方法用于化妆品中多环芳烃的检测,回收率在90.6%～95.9%之间,相对标准偏差均小于5.1%（n=5）。
A sensitive analytical method based on reversed microemulsion electrokinetic chromatography（ MEEKC） combined with on-line preconcentration technique was developed for the determination of polycyclic aromatic hydrocarbons（ PAHs） in cosmetics. For six lipophilic PAHs analytes which are difficult to be separated under conventional conditions,three stacking techniques including large volume sample stacking（ LVSS）,dynamic pH junction and sweeping（ LVSS-DypH-sweep） were combined to realize the efficient preconcentration and separation. Under the optimum conditions,including the microemulsion buffer with the composition of 2. 4%（ w / w） SDS-0. 6%（ w / w） octane-6. 6%（ w / w） n-butyl alcohol-20 mmol / L Na H2PO4（ pH 2. 2）,HCB injection time of 20 s（ 16 kPa） and sample injection time of 80 s（ 16 kPa）,good enrichment effect was reached with the enrichment factors ranged from 25 to 80,and the PAHs were analyzed successfully within 27 min. The developed method was used to analyze the PAHs in cosmetics. The recoveries ranged from 90. 6% to 95. 9%. The RSD values（ n = 5） were less than 5. 1%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第1期81-86,共6页
Chinese Journal of Analytical Chemistry
基金
国家质量检验检疫监督总局资助项目(No.2010IK183)~~
关键词
微乳毛细管电泳
大体积进样
P
H动态连接
扫集
多环芳烃(PAHs)
Microemulsion electrokinetic chromatography
Large volume sample stacking
Dynamic p H junction
Sweeping
Polycyclic aromatic hydrocarbons
