摘要
为了实现对微弱神经递质信号的在体实时检测,设计并实现了一种无线电化学检测仪器。系统硬件以低功耗微控制器MSP430为主控制器,包括了微弱电流检测模块、波形产生模块以及数据收发模块,具有尺寸小（2.3 cm×1.8 cm×0.6 cm）,功耗低的特点。基于u C/OS操作系统设计了系统的下位机程序,结合上位机软件实现了检测数据的实时显示与分析。针对神经递质的实际检测需求,系统集成了计时电流法与快速循环伏安法两种电化学检测分析方法。利用快速循环伏安法对不同浓度的多巴胺标准溶液进行了测试,在浓度范围5.0×10^-7～7.0×10^-5mol/L内,系统检测的响应电流与多巴胺浓度之间线性相关系数R=0.99。在此基础上,开展了大鼠在体多巴胺检测的实验,并成功检测到大鼠尾状壳核脑区的电诱发多巴胺释放信号。实验结果表明,此仪器具有检测灵敏度高的优点,能够实现对大脑内神经递质信号的定性及定量分析,在神经科学研究领域具有广阔的应用前景。
A wireless electrochemical recording device was designed for in-vivo neurotransmitters real-time detection. Low-power microcontroller MSP430 was chosen as main control unit in hardware system. Other modules were current detection module,waveform generator module and data transceiver module. This device had the merits of small size（ 2. 3 cm × 1. 8 cm × 0. 6 cm） and low power consumption. Firmware program design was based on u C / OS operating system. Combined with the PC software,the device could achieve online display and analysis of the recording data. For neurotransmitter detecting needs, the device implemented fast-scan cyclic voltammetry（ FSCV） and fixed-potential amperometry. By using fast-scan cyclic voltammetry method,a linear relationship（ R = 0. 99） between the concentration of dopamine and response current was acquired in the range of 5. 0 × 10^-7- 7. 0 × 10^-5mol / L. In the in-vivo experiments,the electrically evoked dopamine was recorded in the caudate-putamen area of brain in rats. Experimental results showed that the system had high detection accuracy,which could realize qualitative and quantitative analysis of the brain neurotransmitter. This work would have a broad application prospect in the field of neuroscience research.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第1期93-97,共5页
Chinese Journal of Analytical Chemistry
关键词
电化学
神经递质
多巴胺
快速循环伏安
Electrochemistry
Neurotransmitter
Dopamine
Fast-scan cyclic voltammetry
