摘要
建立了超高效合相色谱法（Ultra performance convergence chromatography,UPC^2）分离和测定复合维生素片中11种脂溶性维生素（A,D,E,K）及其衍生物的方法。超高效合相色谱（UPC^2）技术集合超临界流体色谱（Supercritical fluid chromatography,SFC）和超高效液相色谱（Ultra performance liquid chromatography,UPLC^TM）的技术优点,流动相以CO2为主体,乙腈为助溶剂梯度洗脱。选用Waters Acquity UPC2HSS C18SB色谱柱（100 mm×3.0 mm 1.8μm）,流速1 mL/min,检测波长为284 nm。方法检出限在1.5～2.0 mg/L之间;VK1,VK2,VK3和VD3的线性范围分别为3～300 mg/L;VA、VA棕榈酸酯、VA甲酸、VE、VE醋酸酯、VE琥珀酸酯和VD2的线性范围分别为5～300 mg/L;加标回收率范围为97.31%～98.76%;相对标准偏差为0.41%～0.96%,可以满足复合维生素片中11种脂溶性维生素（A,D,E,K）及其衍生物的方法要求。
A new method was developed for the determination of 11 fat-soluble vitamins （A, D, E and K） and its derivatives in vitamin tablets by ultra performance convergence chromatography （UPC^2）. The mobile phase was the mixture of supercritical CO2 and acetonitrile at a flow rate of 1 mL/min. The separation was carried out on the Waters Acquity UPC^2 HSS C18 SB 100 mm× 3.0 mm i. d. , 1.8 μm column. The UV detector was set at a wavelength of 284 nm. The limits of detection （LOD） were 1.5 -2.0 mg/L, and the calibration linear for VK1, VK2, VK3 and VB3 was 3-300 mg/L, linear for VA, VA palmitate, VA formic acid, VE, VE acetate, VD2 and VD3 was 5-300 mg/L, respectively. Its spiked recoveries were 97.31% - 98.76% , and the relative standard deviations （RSDs） were 0. 41% -0. 96%. The method is applicable for the determination of fat-soluble vitamins （A, D, E and K） and Their derivatives in vitamin tablets.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2015年第1期115-120,共6页
Chinese Journal of Analytical Chemistry
关键词
超高效合相色谱
脂溶性维生素
复合维生素片
Ultra performance convergence chromatography
Fat-soluble Vitamin
Vitamin tablets
