摘要
以日本大叶菠菜为材料,采用土培法,研究了不同浓度水杨酸(SA)对盐胁迫下菠菜叶表皮气孔开度的调节作用.利用扫描电镜、普通光学显微镜及蒽酮法对菠菜叶表皮气孔形态、气孔开度及可溶性糖含量进行测试和表征.实验结果表明:随着SA浓度的增大,叶表皮气孔形态由细长线形逐渐变成椭圆形;叶表皮气孔开度逐渐增大,当SA浓度为200 mg/L时,气孔开度最大;叶表皮可溶性糖含量呈先增加后降低的变化趋势,当SA浓度为150 mg/L时,可溶性糖含量达到最大,比盐胁迫下的含量增加了28.9%.因此,SA的加入可以调节菠菜叶表皮的气孔形态和气孔开度,提高其光合效率,改善其渗透调节作用,从而增强菠菜的抗盐性.
Spinacia oleracea L was used as materials to study the effect of salicylic acid（SA）with different concentrations on stomatal aperture of spinach leaf epidermis in the condition of salt stress.Epidermis stom-atal morphology,stomatal aperture and soluble sugar content were characterized and tested by scanning electron microscope（SEM）,ordinary optical microscope and anthrone method.Results presented that with the increase of SA concentration,leaf epidermis stomatal morphology by slender line gradually became oval;the leaf epidermis stomatal aperture gradually increased,when the SA concentration was 200 mg/L, the stomatal aperture achieved maximum;leaf soluble sugar content presented the trend of increase firstly, then lower,when SA concentration was 150 mg/L,soluble sugar content achieved maximum,and the con-tent increased by 28.9%than that under salt stress.The addition of SA could adjust the stomatal morpholo-gy and stomatal aperture of spinach leaf epidermis,and enhance its photosynthetic efficiency and osmotic regulation effect,thus improve the salt resistance of spinach.
出处
《郑州轻工业学院学报（自然科学版）》
CAS
2014年第6期33-38,共6页
Journal of Zhengzhou University of Light Industry:Natural Science
关键词
菠菜
气孔开度
盐胁迫
水杨酸
Spinacia oleracea L
stomatal aperture
salt stress
salicylic acid
