摘要
部分潘那利番茄渐渗系具有抗坏血酸(As A,又名VC)高积累特性,但渐渗片段区间偏大。本试验拟得到与As A相关的渐渗亚系,解析As A积累相关的QTL位点。构建了IL8-1与M82杂交的F2及F2:3家系遗传群体,根据生物信息学软件分析筛选分子标记用于群体的检测,最终得到4个纯合的重组单株,分别命名为48-1(5c M)、281-2(13c M)、559-1(20c M)、281-7(3c M),覆盖IL8-1(22c M)渐渗区间。对亲本及得到的重组单株进行As A含量的测定,结果显示IL8-1总As A含量显著高于M82。渐渗亚系48-1、281-2、559-1的总As A含量均显著高于M82。而亚系281-7的总As A和还原态As A含量都显著低于M82。结合分子标记检测和As A含量测定可以得出,IL8-1中与总As A相关的主效基因在标记U221657和At5g46630之间的基因组区域(5c M),与还原态As A相关的主效基因在At5g27390和At5g46630之间的基因组区域(13c M)。而在Indel-3和CT-92之间(3c M)可能存在As A的负向调控位点。
Part of tomato（Solanum lycopersicum）introgression lines generated from Solanum pennel-lii shows high accumulation characteristics in ascorbic acid（VC）content. However,some of the introgression fragment is too large to dissect to the genetic basis of AsA. We aim to get sub-introgression lines related to QTL of ascorbic acid. We constructed F2 and F2：3 populations derived from IL8-1 and M82,and then detected the F2 and F2： 3 populations using polymorphic markers generated from bioinformatics analysis. Finally,we got 4 sub-introgression lines namely 48-1（5 cM）,281-2（13 cM）,559-1（20 cM）and 281-7（3 cM）,respectively,which covered IL8-1（22 cM）introgression fragment. Determination of ascorbic acid content of sub-introgression lines and their parents showed that the total ascorbic acid content in IL8-1 was significantly higher than that in M82,and the total ascorbic acid content of sublines 48-1,281-2 and 559-1 was also significantly higher than that in M82. While,the content of total ascorbic acid and reduced ascorbic acid in subline 281-7 was remarkably lower than that in M82. Combining molecular marker test and ascorbic acid measurement,we can suggest that the primary loci of total ascorbic acid on IL8-1 locate between markers U221657 and At5g46630（5 cM）,genes related to reduced ascorbic acid is located in the genomic region between At5g27390 and At5g46630（13 cM）,and the genetic loci negatively related to ascorbic acid are probably located between markers Indel-3 and CT-92（3 cM）.
出处
《中国蔬菜》
北大核心
2015年第1期19-23,共5页
China Vegetables
基金
国家自然科学基金项目(31171974)
国家"973"计划项目(2011CB100600)
关键词
抗坏血酸
QTL
渐渗亚系
番茄
QTL
Ascorbic acid
Quantitative trait loci
Sub-introgression line
Tomato
