摘要
目的:研究补中益气汤对甲状腺功能减退(以下简称甲减)大鼠心肌甲状腺激素受体(Thyroid Hormone Receptor,TR)TRα1 mRNA表达的影响。方法:将Wistar大鼠随机分为正常对照组、甲减模型对照组、西药左旋甲状腺素钠片对照组(简称L-T4组)、补中益气汤组,每组30只。正常对照组大鼠予普通饲料,双蒸水;予模型对照组大鼠低碘饲料,双蒸水;L-T4组大鼠予低碘饲料,灌服2mL/d,含量为0.52μg/kg左旋甲状腺素钠片的混悬液,并灌服2mL双蒸水;予补中益气汤组大鼠低碘饲料,灌服2mL/d含生药量为18.9g/kg的补中益气汤煎剂。酶联免疫吸附测定法测大鼠血清促甲状腺激素(Thyroid Stimulating Hormone,TSH),放射免疫分析法测定三碘甲状腺原氨酸(Total Triiodothyronine,TT3),总甲状腺素(Serum Total Thyroxine,TT4)。实时定量聚合酶链反应检测大鼠心肌TRα1 mRNA的表达,获得基因扩增结果后,再用参照基因的△C_T法计算每个样本中参照基因与目的基因的C_T值差异,能衡量基因表达水平。砷铈分光光度法测定尿碘含量。光镜下观察大鼠心肌形态。心电图机记录大鼠心电变化。结果:与模型组相比,L-T4组和补中益气汤组大鼠血清TT3值均明显升高(P<0.01);与模型组相比,L-T4组和补中益气汤组大鼠血清TT4值均也明显升高(P<0.001);L-T4组与补中益气汤组大鼠血清TT3、TT4值比较,两组无明显差异。与模型组比较,L-T4组和补中益气汤组大鼠TSH值明显降低(P<0.01),其中补中益气汤组较L-T4组血清TSH值降低明显。补中益气汤组心率及低电压恢复明显好于L-T4组(P<0.05);补中益气汤组心肌TRα1 mRNA的表达较L-T4组高1.08倍(P<0.05);心肌细胞形态观察发现补中益气汤组较L-T4组恢复得明显。结论:补中益气汤能使甲减大鼠心肌损害恢复得更好,其作用机制与TRα1 mRNA在心肌细胞上的表达有关。
Objective： To study the effect of the Buzhong Yiqi decoction on expression of TRal mRNA in the myocardial of hypothyroidism rats. Methods： Wistar rats were randomly divided into the control group, the model group, the L-T4 group, and the Buzhong Yiqi decoction groups, 30 cases for each. Rats in the control group were treated with ordinary diet and double distilled water （DDW） while rats in the model control group were fed with low iodine diet and DDW. The rats in the L-T4 group to low iodine diet, daily gavage for 2 mL content was 0.52g/kg levothyroxine sodium tablets and suspension, gavage for 2 mL double distilled water; to the Buzhong Yiqi decoction in rats with low iodine diet, daily gavage for 2 mL containing quantity is 18.9g/kg Buzhong Yiqi decoction. Serum thyroid-stimulating hormone （TSH） was measured with enzyme-linked immuno sorbent assay. Serum total thyroxine （TT4） and triiodothyronine （TT3） were measured with radioimmunoassay. Myocardial TRal mRNA was measured with real-time polymerase chain reaction. Gene amplification results, and then reference gene CT method to calculate each sample reference gene and target gene CT difference, can measure the level of gene expression. Get-arsenic cerium spectrophotometry was used to measure the median urinary iodine. Myocardial morphological changes were observed under optical microscope, and myocardial electrocardio-graphic changes were measured by electrocardiograph （ECG）. Results： After giving the processing factor for 56d, TT3 and TT4 values of L-T4 and Buzhong Yiqi decoction groups were higher than normal and model groups （P〈0.01, P〈0.01）. Compared with the model group, the TSH value of the Buzhong Yiqi decoction and L-T4 groups was significantly decreased （P〈0.01）, and the value of the Buzhong Yiqi decoction group was obviously lower than that of L-T4 group. ECG indicates statistical difference between the L-T4 and the Buzhong Yiqi decoction groups （P〈0.05）, and the heart rate and low-volume of rats in the Buzhong Yiqi decoction group were better recovered than those of rats in the L-T4 group. The myocardial TRal mRNA expression in the Buzhong Yiqi decoction group is 1.08 times higher than L-T4 group. Myocardial morphology of the Buzhong Yiqi decoction group was recovered more obviously than L-T4 group. Conclusion： The Buzhong Yiqi decoction can make a better recovery for myocardial injury caused by hypothyroidism due to the mechanism of myocardial TRal mRNA expression.
出处
《中医临床研究》
2014年第35期1-5,共5页
Clinical Journal Of Chinese Medicine
基金
教育部博士点基金(20092133110004)
辽宁省教育厅(2009A492)
辽宁省教育厅优秀人才计划(LR201026)
