摘要
采用2种方法研究了复方糖苷抗新城疫病毒(Newcastle disease Virus,NDV)感染作用。一是鸡胚成纤维细胞(Chicken embryo fibroblast,CEF)培养法,通过观察NDV CPE的形成及测定CEF OD值的变化,计算病毒抑制率等指标,综合评价复方糖苷在细胞水平上抗NDV作用,并从直接灭活病毒、阻断病毒吸附与生物合成及释放等环节,探讨了复方糖苷的抗NDV途径。二是将复方糖苷稀释成1 200,1 000,800,600,300μg/m L 5种浓度与100 EID50 NDV液混合后接种9日龄SPF鸡胚,于72 h后统计鸡胚的存活数并测定尿囊液血凝效价(HA),依此判定复方糖苷在鸡胚内抗NDV作用。结果表明:随着药物浓度的增加CPE的特征逐渐减弱,OD值明显升高,病毒抑制率增大,当复方糖苷质量浓度为600μg/m L时对NDV具有显著的直接杀灭与阻断吸附作用。HA在1 200,1 000μg/m L时均为0。表明复方糖苷具有显著的抗NDV感染作用。
Two methods were used to study the inhibition of compound glycosideon replication of Newcastle Disease Virus in cell and embryo. The first was CEF cell cultural method, and through observation of NDV CPE formation and determination of CEF OD value changes, virus inhibition rate was calculated to comprehensively evaluate NDV inhibition of compound glycoside at the cellular level. From direct inactivated virus, blocking virus adsorption and biosynthesis and release of NDV resistant approach of compound glycoside were discussed. The second： compound glycoside was diluted into five different concentrations, 1 200, 1 000, 800, 600 and 300μg/mL, and, vaccinated to 9day SPF chicken embryos after being mixed with 100 EID50 NDV. After 72 h, survival of chicken embryos was added up and HA titer of allantoic fluid was determined to evaluate antiNDV effect of compound glycoside in chicken embryos. Experimental results showed that with the increase of drug concentration, characteristics of CPE weakened gradually, OD value increased significantly and virus inhibition rate increased, and when concentration of compound glycoside was 600μg/mL , NDV had significant direct killing and block adsorption funtions. In 1 200 and 1 000μg/mL, HA was 0,indcating that compound glycoside had significant antiNDV effect.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2014年第6期713-718,共6页
Journal of Jilin Agricultural University
基金
江苏省自然科学基金项目(BK2008587)
江苏农牧科技职业学院重点项目(NSFZD1305)
