摘要
目的通过检测同一宫颈上皮内癌变或宫颈癌疑似病例宫颈石蜡包埋样本及脱落细胞中HPV E6/E7 m RNA的水平,分析两种检测结果的差异,探讨二者在宫颈癌筛查的应用价值。方法对302例宫颈上皮内癌变或宫颈癌疑似病例分别取宫颈活检组织及脱落细胞,两类标本均采用b DNA技术进行HPV E6/E7 m RNA检测,同时行病理学检查,并对检测的阳性率及灵敏度、特异度做出统计学分析。结果石蜡包埋组织检测HPV E6/E7 m RNA的阳性率为48.7%,高于脱落细胞的阳性率40.1%(χ2=4.192,P<0.05)。石蜡包埋组织检测HPV E6/E7m RNA对诊断的灵敏度为75.0%,高于脱落细胞的61.4%(χ2=5.046,P<0.05);石蜡包埋组织检测HPV E6/E7 m RNA对诊断的特异度为81.2%,脱落细胞为77.1%,二者差异无统计学意义(χ2=0.641,P>0.05)。结论检测石蜡包埋样本中的HPV E6/E7 m RNA,可更加精确预测宫颈病变进展风险,对宫颈癌筛查工作具有更好的临床意义。
Objective To analyze the value of HPV E6/E7mRNA between cervical exfoliated cells and paraffinembedded samples in the same patient and discuss the application value between them in cervical cancer screening. Methods Cervical biopsy tissues and exfoliated cells were collected from 302 samples and bDNA technology was used to detect the presence of HPV E6/E7 mRNA in two kinds of specimens and the positive rate, the sensitivity and specificity were statistically analyzed. Results The positive rate of paraffin-embedded samples was higher than that of cervical exfoliated cells （x^2= 4.192,/9〈0.05）. The sensitivity in diagnosis of HPV E6/E7 mRNA in paraffin-embedded samples was higher that that in cervical exfoliated ceils without significant difference between them in diagnostic sensitivity（x^2 = 0.641, P〉0.05）. Conclusion Detection of HPV E6/E7 mRNA from paraffin-embedded samples and foliated cells is a more accurate method for predicting risk of cervical lesions and it possesses clinical significance for screening of cervical lesions.
出处
《中国热带医学》
CAS
2014年第12期1493-1495,共3页
China Tropical Medicine
