摘要
建立了免疫亲和柱净化-柱前化学衍生-高效液相色谱荧光检测器同时检测谷物中T-2毒素和HT-2毒素的方法。样品经溶剂[V（甲醇）∶V（水）=80∶20]提取,通过免疫亲和柱净化（IAC）,以氰酸蒽（1-AN）为衍生化试剂、4-二甲基氨基吡啶（DMAP）为催化剂进行衍生,以ZORBAX Eclipse XDB苯基柱为分离柱,乙腈-水为流动相进行高效液相色谱分离和检测。在0.005~0.5μg/g内呈良好线性,检出限为0.005μg/g,添加回收率为82.0%~108.0%,RSD〈15.5%。
A method was developed for the determination of T-2＆HT-2 toxin in grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and pre-cloumn chemical derivatization. Samples were extracted with methanol-water（80：20, v/v） , and purified by immunoaffinity columns containing antibodies spe- cific for T-2＆HT-2 toxin. Then derived with 1-anthroylnitrile（1-AN） and 4-dimethylaminopyridine（DMAP）. ZORB- AX Eclipse XDB-phenyl column and mobile phase of acetonitrile-water were used for analysis. Recoveries from the different cereals spiked with T-2＆HT-2 toxin at levels ranging from 0. 005 to 0.5 μg/g were from 82.0% to 108.0% , the relative standard deviations were less than 15.5% and the limit of detection was 0. 005μg/g. This method has a higher clean-up degree, sensitive, stable, low cost and can be applied widely.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第8期190-193,共4页
Food and Fermentation Industries
基金
国家自然科学基金(No.21276047)
中央高校基本科研业务费(No.DC12010103)资助
