摘要
目的:由于酪蛋白糖巨肽(casein glycomacropeptide,CGMP)具有许多生理活性功能和独特营养特性,研究CGMP对小鼠腹腔免疫细胞以及肠黏膜屏障的影响可为其应用于保健食品和医药品提供科学依据。方法:以96只健康昆明雌性小鼠为实验动物,利用流式细胞仪、HE染色和免疫荧光标记技术,分别检测分析了灌胃不同剂量的CGM P(30μg/d和120μg/d)对实验小鼠腹腔吞噬细胞吞噬活性以及肠上皮内淋巴细胞(intraepithellal lymphocytes,IEL)和固有层IgA+浆细胞数量的影响,探讨CGMP改善小鼠腹腔吞噬细胞和肠黏膜屏障功能的作用。结果:CGMP能够显著增加小鼠腹腔吞噬细胞的吞噬能力,灌胃时间越长,作用效果越明显;其中灌胃CGMP的剂量为120μg/d的作用效果优于30μg/d剂量组。且与对照组相比,灌胃CGMP组小鼠十二指肠上皮内淋巴细胞数和固有层IgA+浆细胞数量显著增加(P<0.01)。结论:CGMP可以增强小鼠腹腔吞噬细胞吞噬功能,诱导肠道黏膜免疫应答,增强肠黏膜免疫屏障功能,有望开发为抑制肠道炎症的功能性食品添加剂。
Objective： Casein glycomacropeptide （CGMP） has many physiological functions and unique nutritional properties. This study aimed to explore its effect on peritoneal immune cells and intestinal mucosal barrier in mice. Methods： After being intragastrically given CGMP at doses of 30 and 120 μg/d, respectively, 96 female Kunming mice were tested for phagocytic activity, intestinal intraepithelial lymphocytes （IEL） subpopulations and the number of IgA＋ plasma cells in the lamina propria by flow cytometry, hematoxylin and eosin （HE） staining and immunofluorescence, respectively. Results： CGMP administration to mice resulted in a significant increase in phagocytic activity in a dose- and time-dependent manner. Compared with the control group, CGMP administration also resulted in a significantly higher percentage of intraepithellal lymphocytes （IEL） in the duodenum and a significantly greater number of IgA＋ plasma cells in the lamina propria （P 〈 0.01）. Conclusion： CGMP can enhance phagocytic activity, induce intestinal mucosal immune response and enhance the intestinal mucosal barrier function.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第9期234-240,共7页
Food Science
基金
国家自然科学基金面上项目(30771524
31071522)
关键词
酪蛋白糖巨肽
肠黏膜屏障
吞噬细胞
肠上皮内淋巴细胞
IgA+浆细胞
casein glycomacropeptide
intestinal mucosal barrier
phagocytic activity
intestinal intraepithelial lymphocytes
IgA^＋ plasma cells
