摘要
采用常量法和微量法对苦参中的总生物碱进行提取,比较这2种方法在提取率、试剂用量和提取时间方面的不同.结果表明,微量法的提取效果略高于常量法,其提取率为0.565％;微量法的苦参用量是常量法的10％,所用试剂乙醇、氯仿、氨水只占常量法的30％左右;完成本次实验微量法共需要6.5h,而常量法则需29 h.
Total alkaloids of sophora flavescens were extracted by macromethod and micro- method, and extraction rate of alkaloids, reagent consumption and extracting time were compared. The results showed that the yield of alkaloids by micromethod was 0. 565 %, slightly high- er than the yield of macromethod. Consumption of sophora flavescens and reagents including ethanol, chloroform and aqueous ammonia by micromethod was about 10% and 30% of that by macromethod respectively. Macromethod took 29 h to complete the whole assay, whereas micromethod needed only 6.5 h.
出处
《化学教育》
CAS
北大核心
2015年第2期23-25,共3页
Chinese Journal of Chemical Education
基金
青岛农业大学实验技术研究项目(SYJK12-23)
青岛农业大学博士启动资金(0616)
关键词
微量法
常量法
苦参
生物碱
micromethod
macromethod
sophora flavescens
alkaloid
