摘要
目的 探讨脑出血患者血清细胞纤维连接蛋白（cFN）的表达水平与脑出血的关系.方法 选择2012年4～9月广西壮族自治区南溪山医院收治的脑出血患者25例作为脑出血组,同期选择健康体检者25例作为健康对照组,应用免疫印迹法（Western blot）和酶联免疫吸附测定（ELISA）检测脑出血患者入院后1、4、7d的血清cFN表达水平,同时对应检测健康对照组血清cFN表达水平.结果 Western blot检测结果显示,cFN在脑出血患者的表达水平显著高于健康对照组;ELISA检测结果显示,脑出血组患者1、4、7d血清cFN水平分别为（238±69）、（448±84）、（280±84）μg/L,对照组1、4、7d血清cFN水平分别为（138±30）、（141±40）、（139±15） μg/L,两组不同时点间比较差异有统计学意义（P＜0.05）.相关性分析结果显示cFN表达水平与血肿体积的大小呈正相关（P＜0.05）.结论 脑出血患者血清cFN表达水平升高,cFN表达水平可能与脑出血患者预后有关,但尚需临床大样本的试验研究.
Objective To investigate serum cellular fibronectin（cFN） levels in patients with intracerebral hemorrhage and its correlation with cerebral hemorrhage.Methods A total of 25 cases of cerebral hemorrhage from Guanyang County People＇s Hospital from Apr.2012 to Sep.2012 were included as the hemorrhage group,and another 25 healthy objects were included as control group.cFN express.ion level in serum samples of both groups at 1,4,7 d after the onset of stroke was measured by Western blot and enzyme-linked immunosorbent assay.Results Western blot analysis showed that the cFN expression level in the hemorrhage group was significantly higher than the control group.The enzyme linked immunosorbent assay （ELISA） indicated that levels of serum cFN were significant higher in the hemorrhage group at each time point after onset than those in the control group.The highest level of serum cFN was found at 4 days.The levels of serum cFN in hemorrhage group at 1,4,7 d after onset were：（238 ± 69）,（448 ± 84）,（280 ± 84） μg/L;while in the control group were：（138 ± 30）,（141 ± 40）,（139 ± 15） μg/L,the differences between the two groups at each time point were statistically significant （P 〈 0.05）.Correlation analysis showed cFN level was positively correlated with the brain edema volume （P 〈 0.05）.Conclusion The serum cFN expression level is higher in hemorrhage patients,and may be associated with the prognosis of the patients,though further validation by large clinical samples study is still needed.
出处
《医学综述》
2015年第1期121-123,共3页
Medical Recapitulate
关键词
脑出血
细胞纤维连接蛋白
预后
Intracerebral hemorrhage
Cellular fibronectin
Prognosis
