摘要
在植物的不同生长、发育、繁殖阶段和不同类型细胞中,基因的表达各不相同。近几年发展起来的研究基因差异表达的方法很多,抑制消减杂交(Suppression Subtractive Hybridization)是其中一项基于转录水平,结合消减杂交与PCR技术的分离差异表达基因的方法,其原理可概括为消减同源序列,富集差异序列。该技术报道于1996年,在一个循环过程中分离差异表达基因尤其是低丰度表达的基因,虽然存在一些缺点,但以其较高的灵敏性、容易操作、假阳性率低、重复性好等优点,在分离及其克隆差异表达基因研究方面得到了广泛的应用。近几年,此技术在植物抗病基因的诱导表达方面已得到了应用,目前大多数研究主要集中在文库构建及筛选等方面。文章对抑制性消减杂交(SSH)技术的原理、优缺点及其在植物抗病性研究机制中应用进展进行了综述。
The genes of plants were differently expressed in various phases of growth、development and multiplication and in different cells. In recent years, numerous methods used to investigate the gene differential expression were developed. Suppression Subtractive Hybridization （SSH）, developed in 1996, is a technique with high efficiency for isolation differently expressed genes at transcriptional level, combining subtractive hybridization with PCR, and its principle can be summarized as eliminating homologous sequences and enriching different sequences. SSH is able to isolate the differently expressed genes in one cycle, especially for genes expressed with low abundance. The features that high specificity, simple operation, low false positive rate and good repeatability make it widely applied in the differential expression genes separation despite of some defects. In recent years, SSH has been applied in studies on induced expression of plant disease resistant genes, with most focus on cDNA library construction and screen. The principle, main advantages and defects, and application progress in mechanism study on plants disease resistance were reviewed in this paper.
出处
《内蒙古农业科技》
2014年第5期102-105,共4页
Inner Mongolia Agricultural Science and Technology
基金
现代农业产业技术体系建设专项资金资助(CARS-210102)
关键词
抑制消减杂交
植物
基因差异表达
Suppression subtractive hybridization （SSH）
Plant
gene differential expression
