摘要
为了解杂色龙虾(Panulirus versicolor)线粒体基因组多态位点及遗传变异,对龙虾类的分类研究奠定基础,作者通过常规PCR步移扩增法获得杂色龙虾线的线粒体基因组全序列,分析了其线粒体基因组的基本特征以及基因排列情况,并结合中国龙虾(Panulirus stimposni)、波纹龙虾(Panulirus homarus)线粒体基因组全序列,综合分析了龙虾属线粒体编码基因多态位点及基因变异状况。结果表明:杂色龙虾线粒体基因组全序列为15 700 bp,由13个蛋白质编码基因、22个t RNA基因、2个r RNA基因和D-loop区组成,保持了泛甲壳动物线粒体基因组的原始排列。杂色龙虾线粒体DNA的碱基组成具有AT偏好性,A+T含量为67%,4种碱基的含量分别为(A=34.6%,T=32.1%,C=20.5%,G=12.8%)。预测了杂色龙虾18个t RNA以及2个r RNA的二级结构。龙虾属线粒体基因组中的nad 2、nad 5基因以及D-loop区的多态位点比例较高,适合作为分子标记用于龙虾类系统进化关系、种内多态性以及遗传分化等方面的研究。
The complete sequence of Panulirus versicolor mitochondrial genome was determined by using conventional PCR and conserved primers walking approaches. The basic characteristics and gene arrangement of P. versicolor mito- chondrial genome were analyzed. Combined with the mitochondrial genome of P. stimposni and P. homarus, the mito- chondrial genes encoding polymorphic loci and gene mutation of Panulirus were also analyzed. The results show that the entire mitochondrial genome of P versicolor is 15700 bp in length, consisting of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes and D-loop control area, which maintained the original arrangement of crustacean animal mito- chondrial genomes. The composition of P versicolor mitochondrial DNA base has AT preference, with 67% A ＋ T con- tent and the content of A, T, C, and G was 34.6%, 32.1%, 20.5, % and 12.8% respectively. The secondary structure of 18 tRNA and 2 rRNA of P versicolor was predicted. The percentage of polymorphic loci of Panulirus mitochondrial genome in had 2, had 5 gene and D-loop control region is high, which are suitable for the phylogenetic relationships, intraspecific polymorphism and genetic differentiation research of Panulirus as molecular markers.
出处
《海洋科学》
CAS
CSCD
北大核心
2014年第11期16-23,共8页
Marine Sciences
基金
国家科技支撑计划资助项目(2007BAD29B03)
