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可注射镁合金/硫酸钙成骨复合材料的制备及性能研究 被引量:1

A novel injectable magnesium/calcium sulfate hemihydrate composite cement for bone regeneration
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摘要 目的:本研究采用半水硫酸钙晶体(Calcium sulphate hemihydrate,CSH)与可吸收镁合金(Magnesium alloy s,Mgalloy s)制备出一种可注射的新型复合人工骨材料,并研究其可注射性,机械性能,生物相容性、生物降解等各方面的性能。方法:对比不同比例Mg/CSH复合材料的初、终凝时间、压缩强度、注射特性;采用X线衍射分析(X-r ay diffr action analy sis,XR D)和能量弥散X射线探测器(Ener gy Disper sive X-r ay Detector,EDX)对材料成分进行分析;模拟体内液态环境,测试材料在37℃浸泡环境下的生物活性及在磷酸缓冲盐浸泡下的重量变化以及p H变化;材料表面形态采用扫描电镜(Scaning electron microscopy,SEM)观察;用大肠杆菌抑菌性实验对材料的抗菌性进行检测。结果:与纯CSH组相比,添加Mg的复合材料凝固时间延长,复合材料的可注射性显著提高(纯CSH的可注射比例为43%,20%Mg/CSH为69%)。随着Mg含量的提高,抗压强度方面也有所提高(P<0.05)。XRD及EDX检测结果均证明材料中含有CSH以及Mg晶体。不同复合材料在浸泡环境下的结果表明,Mg的添加对复合材料的p H无影响;降解率方面,在浸泡时间小于21d时,纯CSH与Mg/CSH复合材料的降解速度差异无统计学意义;在浸泡21d后,复合材料的降解性明显优于纯CSH(P<0.05)。SEM检测浸泡前后材料表面,结果表明浸泡对材料差异无统计学意义;20%Mg/CSH及10%Mg/CSH与纯CSH 24h后抑菌性能的差异有统计学意义。结论:添加镁合金的可注射硫酸钙复合材料展示出良好的机械性能,在可注射能力、抗菌性,体内生物相容性方面也表现良好。该材料具有潜在的临床应用价值。 Objective: To explore a promising injectable bone scaffold for bone repair, we reported a novel magne- sium/calcium sulfate hemihydrate (Mg/CSH) composite with improved properties caused by the incorporation of Mg into CSH in this study. Methods: The 10% and 20% Mg/CSH composite and pure CSH cements were prepared and their com- positions were determined by X-ray diffraction (XRD). Comparative investigations of setting time, injectability, compres- sive strength and bioactivity in simulated body fluid (SBF) between the composites and the CSH material were made. Fur- thermore, we also evaluated the antibacterial property and in vivo biocompatibility after implantations of them in nude mouse. Results: The Mg/CSH possessed a prolonged setting time for up to 6.5 min and markedly improved injectability (the largest injectability was 69± 2%) and mechanical property with a maximum value of 18.6± 2.7MPa compared to CSH (P〈0.05). In addition, Mg/CSH samples showed significantly better degradability than CSH in SBF after 21 days of soaking (P〈0.05). Moreover, the antibacterial property of 10% and 20% Mg/CSH composite and pure CSH was significantly in- creased. Conclusions: The results imply that this new injectable bone scaffold exhibits promising prospects for bone repair and has a great potential in bone tissue engineering.
出处 《口腔颌面修复学杂志》 2014年第6期321-327,共7页 Chinese Journal of Prosthodontics
基金 国家自然科学基金青年基金(项目编号:81200819) 第6批博士后特别资助项目(项目编号:2013T60943)
关键词 可注射骨 半水硫酸钙 镁合金 骨组织工程 骨修复 injectable bone CSH magnesium alloy bone tissue engineering bone repair
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