摘要
目的:探讨信号转导和转录活化因子3(signal transducer and activator of transcription 3,STAT3),Bcl-2和基质金属蛋白酶(matrix metalloproteinase,MMP-2)在结肠直肠腺瘤和结肠直肠腺癌中的表达,研究其与结肠直肠腺癌临床病理特征和预后的关系,阐明三者在结肠直肠腺癌的浸润转移、判断预后中的作用。方法:选取我院2011年9月至2013年8月结直肠黏膜慢性炎100例,结肠直肠腺瘤100例,结肠直肠腺癌200例石蜡标本,其中结肠直肠腺癌伴远处转移70例,无远处转移130例,采用Envison二步法检测STAT3,Bcl-2和MMP-2在三种组织中的表达。结果:STAT3,Bcl-2和MMP-2在结肠直肠腺瘤、腺癌的阳性表达率明显高于在结直肠黏膜慢性炎中的阳性表达率,差异有统计学意义(P<0.05);STAT3、MMP-2在结肠直肠腺癌伴有远处转移病例的阳性表达率明显高于没有远处转移的病例,差异有统计学意义(P<0.05);Bcl-2在结肠直肠腺癌伴有远处转移病例的阳性表达率与没有远处转移病例的阳性表达率差异无统计学意义(P>0.05)。结论:STAT3,Bcl-2和MMP-2均与结肠直肠癌的发生,发展有关;STAT3和MMP-2与结肠直肠癌的远处转移相关,可能提示患者预后不良,可作为评价结肠直肠腺癌复发与转移、不良生存预后的候选临床标志。
Objective： To investigate the expressions of signal transducer and activator of transcription factor 3（STAT3）, Bcl-2,and matrix metalloproteinase 2（MMP-2） in colorectal adenomas and adenocarcinomas, and to explore the relationship with the colorectal clinicopathological features and prognosis of intestinal adenocarcinomas, and their roles in the invasion, metastasis and the prognosis of a colorectal cancer. Methods： The samples were selected from our hospital from September 2011 to August 2013, including 100 cases of paraffin-coded colorectal mucosas with chronic inflammation, 100 cases of paraffincoded colorectal adenoma and 200 cases of paraffin-coded colorectal adenocarcinomas（70 cases with metastasis and 130 cases without distant metastasis）. Envison two-step method was used to detect the expressions of STAT3, Bcl-2, and MMP-2 in each sample. Results： The expression of STAT3, Bcl-2, and MMP-2in colorectal adenomas and adenocarcinomas were significantly higher than those in colorectal mucosas with chronic inflammation（P〈 0.05）. The expressions of STAT3 and MMP-2 in colorectal adenocarcinomas with distant metastasis were significantly higher than those without distant metastasis（P〈 0.05）. No significant difference of Bcl-2 expression was observed in colorectal adenocarcinomas with and without distant metastasis（P〉 0.05）. Conclusions： STAT3, Bcl-2 and MMP-2 were associated with the occurrence and development of colorectal carcinoma. STAT3 was associated with the distant metastasis of colorectal carcinoma and might indicate a poor prognosis.STAT3 might be used as a candidate clinical sign for the recurrence, metastasis, and poor survival prognosis of colorectal adenocarcinoma.
出处
《现代生物医学进展》
CAS
2014年第36期7114-7117,共4页
Progress in Modern Biomedicine
基金
云南省教育厅科学研究基金项目(2012Z07)
