摘要
目的对青藏高原不同产地大花红景天Rhodiola crenulata中没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸进行分析研究,建立定量分析方法,为其进一步利用提供依据。方法采用30%乙醇提取样品;Diamonsil C18色谱柱（250 mm×4.6 mm,5μm）;以乙腈-0.3%磷酸溶液为流动相进行梯度洗脱;检测波长275 nm,柱温25℃。结果没食子酸、红景天苷、酪醇、儿茶素、没食子酸乙酯、对香豆酸分别在38.2-382.0 ng/m L（r=0.999 8）、301.0-3 010.0μg/m L（r=0.999 9）、19.8-198.0 ng/m L（r=0.999 6）、17.1-171.0 ng/m L（r=0.999 6）、4.5-45.0 ng/m L（r=0.999 8）、6.38-63.80 ng/m L（r=0.999 4）呈良好的线性关系,加样回收率均符合定量测定要求。结论该方法简便,快速,准确,可用于同时对青藏高原大花红景天中6种活性成分的测定。
Objective To analyze the six active ingredients in Rhodiola crenulata from the different origins of Qinghai-Tibet Plateau,to establish quantitative analysis methods,and to provide a basis for further use.Methods Samples were extracted with 30% ethanol;In Diamonsil C18 column（250 mm × 4.6 mm,5 μm）,gradient elution was carried out with acetonitrile-0.3% phosphoric acid solution as the mobile phase,detection wavelength 275 nm,and column temperature 25 ℃.Results A good linear relationships of gallic acid,salidroside,tyrosol,catechin,ethyl gallate,and coumaric acid were at 38.2—382.0（r = 0.999 8）,301.0—3 010.0（r = 0.999 9）,19.8 —198.0（r = 0.999 6）,17.1—171.0（r = 0.999 6）,4.5—45.0（r = 0.999 8）,and 6.38—63.80 ng/m L（r = 0.999 4）,respectively.Conclusion The method is simple,rapid,accurate,and can be used simultaneously to determine the content of the six active ingredients in R.crenulata from the different origins of Qinghai-Tibet Plateau.
出处
《中草药》
CAS
CSCD
北大核心
2015年第2期276-279,共4页
Chinese Traditional and Herbal Drugs
基金
"973"计划前期研究专项(2012CB722905)
关键词
大花红景天
没食子酸
红景天苷
酪醇
儿茶素
对香豆酸
没食子酸乙酯
Rhodiola crenulata（Hook.f.et Thoms.） H.Ohba
gallic acid
salidroside
tyrosol
catechin
p-coumaric acid
ethyl gallate
