摘要
目的观察金丝桃素对急性弓形虫感染小鼠脾脏CD4+、CD8+T淋巴细胞亚群的影响。方法将40只昆明种雌性小鼠随机分为4组:空白对照组(A组)、弓形虫感染组(B组)、金丝桃素对照组(C组)和金丝桃素治疗组(D组),每组各10只小鼠。除A组和C组外,B、D两组每组小鼠腹腔接种RH株弓形虫速殖子1×103个,建立急性弓形虫感染动物模型。C、D组于感染12h后腹腔注射给药,A、B组同时进行生理盐水腹腔注射,连续7d后,用流式细胞仪检测小鼠脾脏中CD4+、CD8+T淋巴细胞水平。结果C组和D组CD4+T细胞百分率分别为20.57±1.76和23.33±1.82,CD4+与CD8+T细胞比值分别为2.04±0.40和2.29±0.65,A、B组CD4+T百分率及CD4+/CD8+值分别为12.13±2.35、15.96±2.71和1.35±0.30、0.77±0.92,差异有统计学意义(P<0.01);A、B、C、D组CD8+T细胞百分率分别为8.95±0.40、20.74±1.50、10.42±2.43和10.86±3.18,差异有统计学意义(P<0.01)。结论金丝桃素可显著改善急性弓形虫感染小鼠的细胞免疫功能,增强小鼠抗弓形虫感染的能力。
Objective To observe the effect of hypericin on subsets of CD4^＋and CD8^＋T lymphocytes in the spleens of mice with an acute Toxoplasma gondii infection. Methods Forty female Kunming mice were randomly divided into 4groups of 10 mice each：a control group（group A）,agroup infected with T.gondii（group B）,a control group given hypericin（group C）,and an infected group treated with hypericin（D group）.Mice in groups B and D were intraperitoneally infected with 1×103 tachyzoites of the RH strain of T.gondii to establish an animal model of acute T.gondii infection.Groups C and D were treated 12 hours after intraperitoneal injection;groups A and B were injected with a saline solution at the same time.Seven days later,the levels of CD4^＋and CD8^＋T-lymphocytes in the spleens of mice were measured using flow cytometry. Results The level of CD4^＋T cells in group C（20.57±1.76）and group D（23.33±1.82）and the ratio of CD4^＋cells to CD8^＋cells in group C（2.04±0.40）and group D（2.29±0.65）were significantly greater than the level of CD4^＋T cells and the ratio of CD4^＋cells to CD8^＋cells in the other two groups（P〈0.01）.The level of CD4^＋T cells was 12.13±2.35 in group A and 15.96±2.71 in group B and the ratio of CD4^＋cells to CD8^＋cells was 1.35±0.30 in group A and 0.77±0.92 in group B.The level of CD8^＋T cell in B group（20.74±1.50）was significantly higher than that in the other 3groups（P〈0.01）.The percentage of CD8^＋T cells in the four groups was as follows：8.95±0.40 in group A,20.74±1.50 in group B,10.42±2.43 in group C,and 10.42±2.43 in group D. ConclusionHypericin improved cellular immune function in mice with an acute T.gondii infection and it enhanced their ability to fight a T.gondii infection.
出处
《中国病原生物学杂志》
CSCD
北大核心
2014年第12期1091-1093,1097,共4页
Journal of Pathogen Biology
基金
安徽省教育厅重点资助项目(No.KJ2012A201)
蚌埠医学院科技发展基金项目计划(No.Bykf13A03)
关键词
金丝桃素
弓形虫
淋巴细胞
Hypericin
Toxoplasma gondii
T lymphocyte
