摘要
以人工栽培多脂鳞伞(Pholiota adiposa)及滑子蘑(Pholiota microspora)子实体为材料,采用回流提取法分别得到石油醚、二氯甲烷、乙酸乙酯及甲醇提取物,以DPPH与ABTS自由基清除率为指标评价各提取物抗氧化活性,结果表明:多脂鳞伞与滑子蘑不同溶剂的提取物均有清除DPPH自由基能力并在一定范围内呈现量效关系。其中乙酸乙酯提取物对DPPH自由基清除能力最强,多脂鳞伞和滑子蘑乙酸乙酯提取物清除DPPH自由基的IC50值分别为0.29mg/mL和0.21mg/mL;同样乙酸乙酯提取物对于ABTS自由基清除能力最强,在质量浓度为1.0mg/mL时,多脂鳞伞和滑子蘑乙酸乙酯提取物对ABTS自由基清除率分别为60.25%和65.27%。以不同剂量的多脂鳞伞和滑子蘑水提物灌胃小鼠20d,测定血清中IL-2(interleukin-2)的含量,用以评价其免疫功能,结果表明:多脂鳞伞水提物3个剂量组处理的小鼠血清中IL-2表达水平明显高于滑子蘑组及阳性对照组,滑子蘑组的IL-2表达水平稍低于阳性对照组,但均显著高于阴性对照组。
Extracts of powdered Pholiota adiposa and P.microspora fruit bodies were prepared by refluxing for 2 h with four different organic solvents （petroleum ether at 40 ℃,dichloromethane at 50 ℃,ethyl acetate at 55 ℃ and methanol at 60 ℃）,and associated antioxidant activities were determined using DPPH and ABTS free radical scavenging assays. All the P. adiposa and P. microspora extracts exhibited concentration-dependent DPPH free radical scavenging activity;highest activities were recorded with ethyl acetate extracts of both fungi with IC50 values of 0.29 mg/mL and 0.21 mg/mL,respectively.Correspondingnbsp;IC50 values for ABTS-scavenging activity were 0.64 mg/mL and 0.53 mg/mL, respectively. Serum interleukin-2 （IL-2）levels in mice administered intragastrically with low （3.125 mg/kg）,medium （6.25 mg/kg）and high （12.5 mg/kg）doses of aqueous extracts （65 ℃ for 2 h）of P.adiposa and P.microspora fruit bodies for 20 days were determined by ELISA.Serum IL-2 levels were significantly higher in all cases compared with negative controls,and P.adiposa extracts were significantly more stimulatory than P. microspora extracts at the same concentration.
出处
《食用菌学报》
北大核心
2014年第4期71-75,共5页
Acta Edulis Fungi
基金
教育部创新团队项目(编号:IRT1134)的部分研究内容
