摘要
目的:从壳斗科植物中筛选多酚类成分含量高、抗氧化能力强,具开发价值的植物资源。方法:采用Folin-酚法对8种壳斗科植物总酚的含量进行测定,通过1,1-二苯基-2-苦基肼(DPPH)法,2,2-连氮-双-(3-乙基苯并噻唑啉磺酸-6)二铵盐ABTS自由基清除法、Fe2+螯合法和Fe3+还原法等4种方法评价其提取物的抗氧化能力,分析其总酚含量和抗氧化能力的关系。结果:在所选取的8种壳斗科植物中,红锥的总酚含量最高,为10.61%,其次为大叶栎10.09%。综合4种抗氧化能力检测结果,8种植物都有很好的抗氧化能力,其中红锥抗氧化能力最强,抗氧化能力与总酚含量存在着较好的量效关系。结论:8种壳斗科植物中红锥与大叶栎为最具开发成与抗氧化活性相关的保健品及护肤品的资源植物。
Objective：To screen the polypenols with high content and strong antioxidant capacity,and to encourage the development of Fagaceae resources.Method：The total content of polypenols from 8 kinds of Fagaceae plants were determined by Folin-phenol method,and their antioxidant capacities were evaluated by four antioxidant methods,including 1,1-diphenyl-2-pieryl-2-picrylhydrazyl （DPPH） scavenging,2,2-amino-di （3-ethyl-benzothiazoline sulphonic acid-6） ammonium salt （ABTS） radical scavenging,Fe2＋ chelating,and Fe3＋ reducing assays.The relationship between the total polypenols content and antioxidant capacity was analyzed.Result：The Castanopsis hystrix had the highest total polypenols content （10.61％）,and followed by C.fissa （10.09％）.Comprehensive evaluation of antioxidant capacity test results showed that all plants had good antioxidant capacity,and the C.hystrix had the strongest antioxidant capacity.A dose-effect relationship between the antioxidant capacity and the total polypenols content of C.hystrix was found.Conclusion：C.hystrix and C.fissa were the most potential plant resources for the health and skin care products with antioxidant activity.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期65-69,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西自然科学回国基金项目(2014jj CB20002)
广西植物功能物质研究与利用重点实验室项目(ZRJJ2013-7)
西部之光人才培养计划项目(科发人字[2013]165号)
关键词
壳斗科
总酚
含量测定
抗氧化能力
Fagaceae
total polypenols
determination
antioxidant activity
