摘要
目的:观察血府逐瘀汤对冠心病血瘀模型家兔心功能、心肌及血管的影响。方法:36只健康家兔随机分为正常组、假手术组、模型组、血府逐瘀汤低、中、高剂量组,每组6只。模型组采用左冠状动脉放置缩窄环方法,制备冠心病血瘀模型;假手术组开胸但不放置缩窄环;在模型组基础上,术后第4周起,血府逐瘀汤低、中、高剂量组分别按10,20,40 g·kg-1ig,同时,正常组、假手术组、模型组ig等体积生理盐水,全部各组均是1次/d,连续5周。检测心功能、心肌肥厚指数、血管活性因子水平,观察心肌形态学、冠脉血管壁形态学变化。结果:与正常组比较,模型组心功能下降、心肌肥厚指数升高、血管活性因子血管紧张素II(Ang II)、肿瘤坏死因子-α(TNF-α)、活性氧(ROS)水平均升高而一氧化氮(NO)水平降低,差异均有统计学意义(P<0.05,P<0.01);模型组心肌形态学、冠脉血管壁形态学观察结果显示有病理性改变。与模型组比较,血府逐瘀汤低、中、高剂量组均可以不同程度改善心功能,抑制心肌病理改变,恢复血管活性因子水平和血管形态,差异均具有统计学意义(P<0.05,P<0.01)。结论:血府逐瘀汤可能通过减轻冠脉血管氧化损伤,改善心肌供血和病变从而恢复心功能。
Objective：To observe the effects of Xuefu Zhuyu Tang on cardiac function,cardiac muscle and blood vessel in rabbits with coronary heart disease at stage of blood stasis syndrome.Method：Thirty-six healthy rabbits were randomly divided into normal group,sham-operation group,model group,and Xuefu Zhuyu Tang low-dose,middle-dose,high-dose groups six groups with six rabbits in each group.Left coronary artery implanted constrictor ring to establish the model of coronary heart disease at the stage of blood stasis syndrome.The sham-operation group was just opened the chest but not implanted the constrictor ring.On the basis of the model group from the first day of the fourth week after operation the low-dose,middle-dose,high-dose groups were respectively intragastrie administrated （ig） with Xuefu Zhuyu Tang according to 10,20,40 g ·kg^-1,once a day for continuous five weeks while the control group,the model group and the sham-operation group were ig the same volume normal saline.Then to detect the cardiac function,heart index,the levels of vasoactive factors and observe pathological change pictures of cardiac muscle and blood vessel.Result：Compared with the normal group,in the model group cardiac function declined,heart index increased,the levels of angiotensis Ⅱ （Ang Ⅱ）,tumor necrosis factor-α （TNF-α）,reactive oxygen species （ROS） while the level of nithic oxide （NO） reduced （P 〈0.05,P 〈0.01）,meantime,in the model group there were pathological changes of cardiac muscle and blood vessel.Compared with the model group,all of the low-dose,middle-dose,high-dose groups could improve cardiac function,inhibit the pathological changes of cardiac muscle and blood vessel and restore the normal levels of vasoactive factors,which had a statistical significance （P 〈 0.05,P 〈 0.01）.Conclusion：It was included that Xuefu Zhuyu Tang improved the pathological changes of cardiac muscle and blood vessel and restored the cardiac function by alleviating the oxidative injury of coronary artery.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期165-169,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科学研究与技术开发计划(桂科攻1347003-4)
关键词
血府逐瘀汤
冠心病
心功能
Xuefu Zhuyu Tang
coronary heart disease
cardiac function
