摘要
为了解辽宁省仔猪病毒性腹泻病原的流行情况,从而制订有针对性的防控措施,采用RTPCR方法对2013年4月份至2014年3月份辽宁省9个市采集的228份腹泻病料进行猪流行性腹泻病毒(PEDV)、猪传染性胃肠炎病毒(TGEV)、猪轮状病毒(PRo V)的检测,根据采集病料的时间和地点进行统计。结果显示PEDV和TGEV的阳性率分别为37.72%和15.79%,说明辽宁省仔猪病毒性腹泻主要病原是PEDV,其次是TGEV;PEDV和TGEV在辽宁省感染分布广泛,污染程度较高,并且全年持续存在。
To investigate the prevalence of the pathogens of piglet viral diarrhea in Liaoning province, and make a targeting measure for disease prevention and control. The RT - PCR method was used to detect the pathogens of porcine epidemic diarrhea ( PEDV ), porcine transmissible gastroenteritis virus ( TGE), and porcine rotavirus (PRoV) for the 228 copies of the pathological materials with diarrhea, which were collected from 9 cities of Liaoning province from April in 2013 to March in 2014. The statistical analysis was performed according to the time and place of the collected materials. The results showed that the positive rates of PEDV and TGEV were 37.72% and 15.79%, respectively. The results in- dicate that the main pathogen of piglet viral diarrhea in Liaoning province was PEDV, followed by TGEV. The infections of PEDV and TGEV are widely distributed in Liaoning province, which have high levels of pollution, and continuously exist in the whole year.
基金
国家农业科技成果转化资金项目(2012B0003018)
辽宁省农业科技攻关重点项目(2011214001)