摘要
目的:筛选高良姜抗胃溃疡有效部位,探讨抗溃疡作用机理。方法:将高良姜提取分离为不同部位,观察各部位对无水乙醇、阿司匹林诱导的小鼠胃溃疡模型溃疡指数、胃组织病理切片、血浆胃泌素(GAS)、白介素1(IL-1)、环氧酶2(COX-2)、肿瘤坏死因子(TNF-α)、前列腺素E2(PGE2)的影响。结果:与模型组小鼠比较,高良姜水提物、80%乙醇洗脱物可以显著减少胃溃疡模型小鼠的胃溃疡指数,显著降低血浆GAS、IL-1、TNF-α浓度,显著升高血浆COX-2、PGE2浓度,30%乙醇洗脱物、95%乙醇洗脱物对溃疡指数无显著影响,但可调节血浆GAS、IL-1、PGE2浓度。结论:高良姜各提取分离部位中水提物、80%乙醇洗脱物具有较好的抗胃溃疡效果,抗溃疡作用可能通过减少血浆GAS、IL-1、TNF-α浓度,提高血浆COX-2、PGE2浓度产生。
Objective： To discuss effect of different extraction parts of galangal on experimental gastric ulcer. Methods：Different parts of galangal were extracted and separated. Gastric ulcer in mice was induced by ethanol and aspirin. The effect of different extraction parts of galangal on gastric ulcer index, stomach tissue pathological slices, plasma gastrin-releasing （GAS）, interleukin 1 （IL - 1） and cyclooxygenase 2 （COX-2）, tumor necrosis factor （TNF alpha）, prostaglandin E2 （PGE2） were observed. Results： Compared with model group, water extract and 80 %ethanol elution could significantly reduce the gas- tric ulcer index, significantly reduce plasma GAS, IL - 1, and concentration of TNF alpha. They could also significantly elevat- ed plasma COX-2, concentration of PGE2. 30 % ethanol elution content, 95% ethanol elution content had no significant effect on ulcer index, hut they could regulate GAS plasma, IL - 1, the concentration of PGE2. Conclusions： Water extract, 80% ethanol elution of galangal content has good resistance effect on gastric ulcer probably by reducing the plasma GAS, IL - 1, the concentration of TNF alpha, raising levels of serum cox-2, PGE2.
出处
《海南医学院学报》
CAS
2015年第2期158-160,共3页
Journal of Hainan Medical University
基金
海南省重大科技专项(ZDZX2013008-2)
海口市重点科技计划项目(2014030)~~
