摘要
目的观察枸杞多糖对体外培养的大鼠睾丸支持细胞的增殖作用。方法选8-9 d龄的大鼠,采用两步酶消化法获取原代大鼠睾丸支持细胞,加入不同质量浓度的枸杞多糖,采用MTT比色法、直接细胞计数及CFSE荧光标记,检测不同质量浓度枸杞多糖对体外培养别的睾丸支持细胞增殖的影响。结果与对照组相比,经枸杞多糖处理后的睾丸支持细胞数量明显增多,并呈剂量依赖性。结论枸杞多糖能够促进大鼠睾丸支持细胞体外增殖。
Objective： To detect the effect of lycium bararum polysaccharides（LBP） on the proliferation of sertoli cells（SCs） cultured in vitro. Methods： Primary SCs were separated from 8-9 days male mice by two-step enzyme digestion method. SCs were cultured with LBP at different concentrations in vitro. The quantity of live cells was determined by MTT assay,direct cell counting and fluorescence intensity methods. Results： Compared with the control group,cell viability was significantly promoted after treatment with LBP and the percentage of cell survival in a dose dependent manner. Conclusion： LBP promoted SCs proliferation both in concentration-effect manner in vitro.
出处
《中国中医急症》
2015年第1期35-37,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
宁夏回族自治区自然科学基金资助项目(NZ13187)
关键词
枸杞多糖
睾丸支持细胞
体外
细胞增殖
Lycium barbarum polysaccharides
Sertoli cells
Vitro
Proliferation
