摘要
目的比较沉淀集菌法、罗氏培养法和聚合酶链反应(PCR)方法检测结核分枝杆菌的临床价值。方法收集2012年6月至2013年5月张家港市第一人民医院临床确诊且经抗结核治疗两月后结核患者的痰标本102份,用以上3种方法进行平行检查。结果 102份标本中,沉淀集菌法检测结果阳性标本92份,阳性率90.19%,罗氏培养法阳性48份,阳性率47.06%,PCR法阳性77份,阳性率75.49%。沉淀集菌法与罗氏培养法的符合率为56.86%,而PCR法与沉淀集菌法符合率为81.37%,与罗氏培养法符合率为61.76%。结论 3种方法联合运用,可弥补单独采用一种方法阳性率低的缺点,从而提高检测的准确度。
Objective To evaluate the clinical value of centrifugal precipitation,Roche culture method and polymerase chain reaction （PCR） for the detection of Mycobacterium tuberculosis. Methods One hundred and two sputum specimens were collected from patients with tuberculosis who had been treated for two months from June 2012 to May 2013. Those specimens were used to detected for Mycobacterium tuberculosis by three methods,and the results from three methods were compared. Results In the 102 sam- pies,the numbers of positive result for Mycobacterium tuberculosis detection were 92（90. 19%）,48 （47.06%） and 77（75.49%） with centrifugal precipitation,Roche culture method and PCR respectively. The coincidence rate of results by precipitation and cul- ture method was 56.86 % ,81.37 % by precipitation method and PCR,and 61.76 % by PCR and culture method. Conclusion Combi- nation of the three methods could improve the accurate rate.
出处
《国际检验医学杂志》
CAS
2015年第2期148-149,共2页
International Journal of Laboratory Medicine
基金
国家科技重大专题项目(2013ZX10004905-002)
关键词
结核杆菌
沉淀集菌法
罗氏培养法
聚合酶链反应
Mycobacterium tuberculosis
centrifugal precipitation
roche culture method
polymerase chain reaction
