摘要
为了建立了一种等度洗脱茶叶中6种儿茶素类没食子儿茶素（GC）、表没食子儿茶素（EGC）、儿茶素（C）、表没食子儿茶素没食子酸酯（EGCG）、表儿茶素没食子酸酯（ECG）、表儿茶素（EC）及限量成分咖啡因（CAF）的高效液相色谱（HPLC）定量测定的方法,采用汉邦Benetnach TM C18（5μm×4.6mm×250mm）色谱柱,流动相为甲醇∶水∶磷酸（28∶72∶0.1,v/v/v）,流速为0.8m L·min-1,UV检测波长为280nm,柱温为30℃。结果表明GC、EGC、C、EGCG、ECG、EC和CAF分别在0.10～1.00、0.16～1.00、0.10～1.00、0.05～0.50、0.10～1.50、0.10～2.00和0.02～1.00mg·m L-1内线性关系良好,相关系数在0.9984～0.9999间;平均加样回收率为100.89%（EGCG）,99.07%（CAF）;样品分析中,除GC的分离度RS〉1外,其他6个成分RS均〉2。该方法简单、快速、准确、重现性好,可用于儿茶素类和咖啡因的定量检测。
The contents of six catechins （GC, EGC,C, EGCG, ECG and EC） and limited composition caffeine were investigated by HPLC method with Hanbon BenetnachTM C18 column（5μm×4.6mm×250mm） at 30℃ and the UV detection wavelength was 280nm. The mobile phase was methanol-water-phosphorus acid （28：72：0.1, v/v/v） isocratic elution system,and the flow rate was 0.8mL. min-1. The results showed that the linear ranges of GC,EGC,C,EGCG,ECG,EC and caffeine were 0.10-1.00,0.16-1.00,0.10～1.00,0.05～0.50,0.10～1.50,0.10- 2.00 and 0.02-1.00mg. mL-1 respectively, and the correlation coefficients were ranged from 0.9984～0.9999. The average recoveries of the two most abundant compositions EGCG and CAF were 100.89% and 99.07%, respectively. The resolutions of the six object components were more than 2,except GC. The method was simple, rapid,accurate, reproducible and ideal for routine analysis of catechins and caffeine.
出处
《食品工业科技》
CAS
CSCD
北大核心
2015年第4期53-56,共4页
Science and Technology of Food Industry
基金
“十二五”国家科技支撑计划项目(2011BA106B06)
中央高校基本科研业务费专项资金资助(GK201303009)
