摘要
目的:初步探讨B7阻断剂CD28与细胞毒T淋巴细胞相关抗原4免疫球蛋白(CTLA-4Ig)对B6.MRL-Faslpr/J狼疮小鼠脾脏Th17和调节性T细胞(Treg细胞)表达的影响与其对小鼠狼疮样病征干预作用之间的相关性。方法:将4个月龄大小雌性B6.MRL-Faslpr/J狼疮小鼠16只,随机分为观察组(Ⅰ组)和对照组(Ⅱ组),分别静脉注射CTLA-4Ig及等量PBS,检测小鼠干预前后24 h尿蛋白、ANA抗体、ds-DNA抗体及干预结束2周后血清IL-17A、脾脏中Th17细胞和Treg细胞百分比。结果:末次干预2周后Ⅰ组的24 h尿蛋白、血清ANA及ds-DNA较Ⅱ组下降均有统计学意义(均P<0.05)。末次干预2周后Ⅰ组血清中IL-17A、脾脏Th17细胞比例均较Ⅱ组低,而脾脏的Treg细胞占CD4+T淋巴细胞的比例高于Ⅱ组,差异均有统计学意义(均P<0.05)。结论:CTLA4-Ig具有减轻B6.MRL-Faslpr/J狼疮鼠狼疮样病征的作用;上调Treg细胞、下调Th17细胞可能是CTLA-4Ig减轻B6.MRL-Faslpr/J狼疮鼠狼疮样病征的重要机制之一。
Objective： To observe the correlation between the B7 blocker,CD28 and cytotoxic T lymphocyte-associated antigen4 immunoglobulin（ CTLA-4Ig） impacting on Th17 and Treg cells expressed in the spleen of lupus mice and its intervention role in lupus-like symptoms of B6. MRL-Faslpr / J lupus mice. Methods： Sixteen 4-month-old female B6. MRL-Faslpr / J mice were selected and randomly divided into treatment group（ group Ⅰ） and control group（ group Ⅱ）; injected the same amount of CTLA-4Ig and PBS intravenously,checked their 24 hour urine protein,ANA antibody,ds-DNA antibodies before and after the intervention. Two weeks after the intervention,detected serum IL-17 A,and the percentage of Th17 and Treg cells in their spleen. Results： Two weeks after the last intervention,24- hour urine protein,serum ANA and ds-DNA in group Ⅰ decreased,and all the differences were statistically significant（ P〈 0. 05） compared with group Ⅱ. Two weeks after the last intervention,serum IL-17 A and the proportion of Th17 cells in the spleen in group Ⅰ were lower than those in group Ⅱ,but Treg cells in CD4^＋T lymphocytes was higher than that in groupⅡ,all the differences were statistically significant（ P〈 0. 05）. Conclusion： CTLA4-Ig can relieve the lupus-like symptoms in lupus mice; raising the number of Treg cells and decreasing the number of Th17 cells may be one of the important mechanisms for CTLA-4Ig to alleviate lupus-like symptoms in B6. MRL-Faslpr / J mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第1期40-44,共5页
Chinese Journal of Immunology
基金
广西自然科学基金重点项目(2013GXNSFA253001)
广西自然科学基金项目(2010GXNSFA013186)
广西卫生厅重点课题(桂卫重200829)
