摘要
采用活性炭对板蓝根多糖进行了脱色研究。在单因素实验的基础上进行了正交实验,得到活性炭脱色的最佳工艺条件为:70℃下调节p H为7.0,添加体积分数为1.0%的活性炭,搅拌80 min,脱色率为92.97%、多糖保留率为92.45%。采用DPPH孤电子配对法测定DPPH自由基的清除作用;采用邻苯三酚自氧化法检测超氧阴离子自由基的清除作用。实验结果表明,板蓝根多糖具有一定的清除自由基的能力,但脱色后的多糖清除自由基的能力降低。
In this study,the experimental condition for decolorization of polysaccharide from Isatidis indigotica was optimized. Based on single factor experiments,orthogonal experiment was performed to select the optimal condition for the decolorization of polysaccharide using the decolorization rate and polysaccharide retention rate as indexes. The optimal technological process of decolorization was adding 1. 0%（ m / v） active carbon to polysaccharide solution at 70 ℃,with p H at 7. 0 and decoloring the solution for 80 min. The decolorization rate was 92. 97%,and the retention rate of polysaccharide was 92. 45%. DPPH lone electron conjugate method was used to detect scavenging effect of DPPH radical; pyrogallol autoxidation method was used to detect scavenging effect of superoxide anion radical. The results indicated I. indigotica polysaccharides had certain ability of scavenging free radicals,but the scavenging ability was decreased after decolorization.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2014年第12期2041-2045,2021,共6页
Natural Product Research and Development
基金
国家自然科学基金(81073023)
江苏省产学研联合创新奖金项目(BY2009109)
南京中医药大学"康缘中医药科技创新基金"(HZ0808KY)
江苏高校优势学科建设工程(YSXK-2010)
江苏省2013年度普通高校研究生科研创新计划(CXZZ13 0631)
关键词
板蓝根
多糖
脱色
活性炭
抗氧化
Isatidis indigotica
polysaccharide
decoloration
active carbon
antioxidation
